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Automation of chemiluminescence determination of ATP by reaction with luciferaze
Čechurová, Petra ; Chocholouš, Petr (advisor) ; Sklenářová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical chemismy Candidate: Bc. Petra Čechurová Supervisor: PharmDr. Petr Chocholouš, Ph.D Title of diploma thesis: Automation of chemiluminescence determination of ATP by reaction with luciferase This thesis deals with the development of methods for automating the chemiluminescence determination of ATP by reaction with luciferin-luciferase system. The aim was to test the possibilities to replace the original manual batch method with SIA method. Analyses were performed by sequential injection analysis with two manners of detection. The detector with a spiral flow cell and the detector with a discontinuous flow cell to monitor reaction kinetics (values of chemiluminescence were readed at time 25, and 30 s) were used. Two different volumes (10 and 20 μl) of injected sample were used within the analyses. The calibrations were measured and calibration curves were calcuated with correlation coefficients of 0.9994 and 0.9894 for the first detector and 0.9953, 0.9951 and 0.9982, 0.9972 for the second detector. The repeatability of the method, calcuated as relative standard deviations were ranged up to 6%. The results of samples with known concentration matched the calibration curves with variations probably caused by high...
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Basic relationship between method administration accounting and processing of budget. It will be demonstrated on the example of chosen non - profit organization
ČECHUROVÁ, Petra
Subject of this thesis is {\clqq}Basic relationship between method administration accounting and processing of budget. It will be demonstrated on the example of chosen non - profit organization.{\crqq} The aim of thesis is to point out connection between setting and checking the budget and method of leading accountancy. At first is written teoretical part of work, where is characterized general way of leading accountancy, basic characteristic of processing the budget, analysis of chosen information needed for processing the budget in accountancy scope. The second part is practical, where is described chosen non-profit organization and features of way, how to lead accountancy, documentation of processed budgets and metodical proposal for their processing. Connection between budgets and accountancy does exist. To compile the budget is closely connected with information from accountancy. Organization come out from accountancy schedule, during the process of puting together budget structure. It depends on who is puting the budget together. Every municipality has its own employee, who is engaged in this and also sets appearance arrangement according to his subjective consideration and according to survey of information for user. The budget comes from accountancy and is arranged with budget changes. The most economical seems to use the possibility of leading only one bank account, which is account 231 - The basic current account in connection with volume of property, budget, number of operations and activities etc.
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