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A bioconversion study of cellulosic waste to ethanol using yeasts systems
Čalová, Iveta ; Vítová, Eva (referee) ; Babák, Libor (advisor)
This diploma thesis deals with the optimization of the production of ethanol from waste paper using yeast. There were used 4 kinds of paper as a substrate - office paper, non-recycled workbook, recycled workbook and newspaper. All papers were pretreated with the following procedures: grinding, microwaves + NaOH, microwave + H2SO4 and microwave + H2SO4 + NaOH. The glucose concentration was determined in enzymatic hydrolysis by HPLC. Saccharomyces cerevisiae were chosen for ethanol production. The production of ethanol was carried out with all the pretreated papers in simultaneous saccharification and fermentation. During hydrolysis, the pretreated papers have reached the highest results in the combination with microwave + H2SO4 + NaOH. Non-recycled workbook was the only exception, where the highest concentration of glucose has been obtained by the pretreatment of microwaves + H2SO4. Following results have been acquired: office paper 24,69 gdm-3, non-recycled workbook 22,47 gdm-3, recycled workbook 16,94 gdm-3 and newspapers 15,36 gdm-3. SSF was carried out again with all the papers and their pretreatments. The highest concentration of ethanol has been achieved in microwave pretreatment + H2SO4 + NaOH. The highest overall concentration has been gained from the office paper, amounted to 16,98 gdm-3. The maximum concentration of ethanol for non-recycled workbook has been 15,25 gdm-3, for recycled workbook 12,2 gdm-3 and for newspapers 12,59 gdm-3.
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Application of Zymomonas mobilis for bioethanol production from cellulose materials
Čalová, Iveta ; Zichová, Miroslava (referee) ; Babák, Libor (advisor)
The thesis deals with the use of Zymomonas mobilis to produce bioethanol from waste paper. There were used three types of paper made by various manufacturers to optimize the production of bioethanol. Individual papers were subjected to the same adjustments namely mechanical grinding and subsequent enzymatic hydrolysis. During cultivation of Zymomonas mobilis in different hydrolysates were taken samples for determination of bioethanol production. The analysis was carried out on high-performance liquid chromatography. Production of bioethanol and glucose consumption was evaluated as well as production of lactic acid since it was also noticeable on the chromatograms.
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A bioconversion study of cellulosic waste to ethanol using yeasts systems
Čalová, Iveta ; Vítová, Eva (referee) ; Babák, Libor (advisor)
This diploma thesis deals with the optimization of the production of ethanol from waste paper using yeast. There were used 4 kinds of paper as a substrate - office paper, non-recycled workbook, recycled workbook and newspaper. All papers were pretreated with the following procedures: grinding, microwaves + NaOH, microwave + H2SO4 and microwave + H2SO4 + NaOH. The glucose concentration was determined in enzymatic hydrolysis by HPLC. Saccharomyces cerevisiae were chosen for ethanol production. The production of ethanol was carried out with all the pretreated papers in simultaneous saccharification and fermentation. During hydrolysis, the pretreated papers have reached the highest results in the combination with microwave + H2SO4 + NaOH. Non-recycled workbook was the only exception, where the highest concentration of glucose has been obtained by the pretreatment of microwaves + H2SO4. Following results have been acquired: office paper 24,69 gdm-3, non-recycled workbook 22,47 gdm-3, recycled workbook 16,94 gdm-3 and newspapers 15,36 gdm-3. SSF was carried out again with all the papers and their pretreatments. The highest concentration of ethanol has been achieved in microwave pretreatment + H2SO4 + NaOH. The highest overall concentration has been gained from the office paper, amounted to 16,98 gdm-3. The maximum concentration of ethanol for non-recycled workbook has been 15,25 gdm-3, for recycled workbook 12,2 gdm-3 and for newspapers 12,59 gdm-3.
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Application of Zymomonas mobilis for bioethanol production from cellulose materials
Čalová, Iveta ; Zichová, Miroslava (referee) ; Babák, Libor (advisor)
The thesis deals with the use of Zymomonas mobilis to produce bioethanol from waste paper. There were used three types of paper made by various manufacturers to optimize the production of bioethanol. Individual papers were subjected to the same adjustments namely mechanical grinding and subsequent enzymatic hydrolysis. During cultivation of Zymomonas mobilis in different hydrolysates were taken samples for determination of bioethanol production. The analysis was carried out on high-performance liquid chromatography. Production of bioethanol and glucose consumption was evaluated as well as production of lactic acid since it was also noticeable on the chromatograms.
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