Národní úložiště šedé literatury Nalezeno 2 záznamů.  Hledání trvalo 0.00 vteřin. 
Cytostatický efekt nostatinu A a jeho přírodních analogů na buněčnou linii HeLa
VICKOVÁ, Kateřina
Cyanobacterial secondary metabolites are a rich source of bioactive compounds with potential utilization in pharmacology. The aim of this study was to evaluate the effect of the novel compound nostatin A isolated from the cyanobacterium Desmonostoc muscorum. Project was focused on the extraction, purification and characterization of the cytostatic effect caused by this novel compound and its naturally occurred structural analogues. The cytostatic activity of nostatin A and its analogs was evaluated in HeLa cell line. Experiments based on microscopy, flow cytometry and HPLC-HRMS techniques were performed in order to clarify the cytostatic effect of nostatin A in HeLa cells and its mechanism of the action.
Cytotoxické působení sinicových metabolitů na vybrané lidské rakovinné linie se zaměřením na pankreatickou linii PaTu
VICKOVÁ, Kateřina
Cancer is disease that affects human population for many thousands of years. Fortunately fighting cancer is more and more effective. This is mainly thanks to combining different strategies of therapy and progress in novel drug development and research. Cyanobacteria are emerging as an important source of novel bioactive secondary metabolites which provides an opportunity for new drug discovery. The aim of this study was evaluation of three cyanobacterial strains for in vitro cytotoxicity against human cancer cell lines, with special focus on pancreatic cancer cells. Crude extracts and chromatographic fractions were tested for their possible use (effectiveness) as selective cytotoxic and/or pro-apoptotic agents. Probably novel compound with anti-proliferative activity was found and isolated by HPLC-MS techniques.

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