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Issues of the algae Chlorella production in flow bioreactors
Jankovičová, Kristína ; Lukavský,, Jaromír (oponent) ; Svěrák, Tomáš (vedoucí práce)
Microalgae invite the attention of scientists due to their unique properties, including their quick growth, accumulation of lipids and other valuable substances, fixation of carbon dioxide and treatment of wastewater. This master´s thesis is focused on the study of microalgae. The main goal is to understand and describe the process of microalgae cultivation, in order to optimize it. The theoretical part of this thesis deals with microalgae (mainly Chlorella sp.) characterization, its practical use and cultivation optimization in order to obtain the highest concentration of biomass. The experimental part is divided into three tasks. Aim of the first task was the comparison of the course of autotrophic and heterotrophic cultivation of various strains of Chlorella and Coccomyxa microalgae, using three different cultivation media – synthetic medium for chlorella cultivation and natural fertilizer, Florium, used in two different concentrations (diluted 50 and 20 times). The highest Chlorella sp. biomass concentration of 7,10 g/l was achieved in the synthetic heterotrophic medium. Second task was focused on monitoring of the growth of algae Coccomyxa and Chlorella strain C1A, with respect to temperature and light intensity, using various combinations of these two important growth factors. Chlorella achieved its highest concentration of 11,46 g/l when grown at temperature of 33,5 °C and light intensity of 320 µE.m2.s1. The third and final task was to observe the growth of Dictiosphaerium chlerelloides microalgae on a flat cascade bioreactor. The experiment led to the discovery that these algae were able to grow at temperatures of around 10 °C, at which many well-known commercial algae, such as Chlorella sp. or Arthrospina sp., simply wouldn’t grow.
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Problematika produkce řas rodu Chlorella v průtočných bioreaktorech
Jankovičová, Kristína ; Kaštánek,, Petr (oponent) ; Svěrák, Tomáš (vedoucí práce)
Bakalárska práca je zameraná na kultiváciu rias rodu chlorella. Teoretická časť práce pojednáva o charakteristike mikroriasy, jej využití a rôznych spôsoboch kultivácie. Zameriava sa na najdôležitejšie faktory ovplyvňujúce jej rast a porovnáva výsledky rôznych výskumov. V experimentálnej časti práce bol pozorovaný rast riasy v rôznych podmienkach. Bol porovnávaný priebeh kultivácie v autotrofnom a heterotrofnom médiu. Ďalej bol sledovaný rast organizmu v závislosti od teploty a intenzity svetla. Pokus bol prevedený v rôznych kombináciách týchto dvoch rastových faktorov. Výsledky experimentu boli zhodnotené pomocou stanovenia dosiahnutej koncentrácie biomasy a merania absorbancie za použitia spektrofotometrickej metódy.
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Issues of the algae Chlorella production in flow bioreactors
Jankovičová, Kristína ; Lukavský,, Jaromír (oponent) ; Svěrák, Tomáš (vedoucí práce)
Microalgae invite the attention of scientists due to their unique properties, including their quick growth, accumulation of lipids and other valuable substances, fixation of carbon dioxide and treatment of wastewater. This master´s thesis is focused on the study of microalgae. The main goal is to understand and describe the process of microalgae cultivation, in order to optimize it. The theoretical part of this thesis deals with microalgae (mainly Chlorella sp.) characterization, its practical use and cultivation optimization in order to obtain the highest concentration of biomass. The experimental part is divided into three tasks. Aim of the first task was the comparison of the course of autotrophic and heterotrophic cultivation of various strains of Chlorella and Coccomyxa microalgae, using three different cultivation media – synthetic medium for chlorella cultivation and natural fertilizer, Florium, used in two different concentrations (diluted 50 and 20 times). The highest Chlorella sp. biomass concentration of 7,10 g/l was achieved in the synthetic heterotrophic medium. Second task was focused on monitoring of the growth of algae Coccomyxa and Chlorella strain C1A, with respect to temperature and light intensity, using various combinations of these two important growth factors. Chlorella achieved its highest concentration of 11,46 g/l when grown at temperature of 33,5 °C and light intensity of 320 µE.m2.s1. The third and final task was to observe the growth of Dictiosphaerium chlerelloides microalgae on a flat cascade bioreactor. The experiment led to the discovery that these algae were able to grow at temperatures of around 10 °C, at which many well-known commercial algae, such as Chlorella sp. or Arthrospina sp., simply wouldn’t grow.
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Problematika produkce řas rodu Chlorella v průtočných bioreaktorech
Jankovičová, Kristína ; Kaštánek,, Petr (oponent) ; Svěrák, Tomáš (vedoucí práce)
Bakalárska práca je zameraná na kultiváciu rias rodu chlorella. Teoretická časť práce pojednáva o charakteristike mikroriasy, jej využití a rôznych spôsoboch kultivácie. Zameriava sa na najdôležitejšie faktory ovplyvňujúce jej rast a porovnáva výsledky rôznych výskumov. V experimentálnej časti práce bol pozorovaný rast riasy v rôznych podmienkach. Bol porovnávaný priebeh kultivácie v autotrofnom a heterotrofnom médiu. Ďalej bol sledovaný rast organizmu v závislosti od teploty a intenzity svetla. Pokus bol prevedený v rôznych kombináciách týchto dvoch rastových faktorov. Výsledky experimentu boli zhodnotené pomocou stanovenia dosiahnutej koncentrácie biomasy a merania absorbancie za použitia spektrofotometrickej metódy.
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