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The Separation of Gastric Aspartic Proteases Using Affinity Chromatography
Frýdlová, Jana ; Kučerová, Zdenka (advisor) ; Barthová, Jana (referee) ; Jonáková, Věra (referee)
Human gastric juice contains mainly aspartic proteases - pepsin A and pepsin C. Both pepsins are produced by gastric mucosa as inactive pepsinogens (pepsinogen A and pepsinogen C) that differ in their physico-chemical and immunological properties. Both pepsinogens consist of molecular variants, isozymogens. Pepsinogens are activated to the corresponding pepsins in the acidic environment of the gastric lumen. (...) A subject of this Ph.D. thesis is a part of a long-term investigation that focuses on the elaboration of methods for the separation of gastric aspartic proteases that would be suitable for monitoring of their changes in mentioned diseases. This thesis was mainly focused on preparation of affinity sorbents suitable for separation of pepsins and pepsinogens. The choice of ligands was based on the substrate N-acetyl-L-phenylalanyl-3,5-diiodo-L-tyrosine that is used to differentiate pepsin A and pepsin C. The following three affinity sorbents were prepared: iodinated L-tyrosine-Sepharose, 3,5-diiodo-L-tyrosine- Sepharose, and N-acetyl-L-phenylalanine-Sepharose. The basic characteristics of the prepared affinity sorbents were determined using the model enzyme (porcine pepsin A). The comparison of the chromatographic behavior of porcine pepsin A and its complex with pepstatine A showed that the enzyme...
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The History of the Pharmacies of Prior Region Rychnov nad Kněžnou
Symonová, Monika ; Král, Karel (advisor) ; Kučerová, Zdena (referee)
Závěr Cílem předkládané diplomové práce bylo podat celkový obraz o historii i současné situaci lékáren na Rychnovsku tak, aby poskytla základní informace nejen dnešním zájemcům o dějiny lékárenství, ale aby též mohla být zdrojem poznatků do budoucna. Na začátku byly stručně zpracovány dějiny Rychnovska ukazující vývoj od dob zemědělství po zavedení průmyslu a rozmach regionu, jako významné a hojně navštěvované kulturní a turistické oblasti. Podrobněji pak byly rozepsány dějiny jednotlivých obcí a měst, kde lze konkrétně vidět postupný vývoj a zejména dnešní situaci jasně ukazující na potřebu lékárny v dané obci či městě. Zachytit historii starých historických lékáren se podařilo na základě studia historických materiálů (metodika zpracování je v předešlé kapitole). Roku 1980 bylo na území bývalého okresu Rychnov nad Kněžnou 9 lékáren. V současnosti existuje v okrese Rychnov nad Kněžnou 24 lékáren. Již tento údaj ukazuje oprávněnost zpracování diplomové práce, navazující na již dříve zpracované materiály. Bez diplomové práce by dějiny 24 lékáren nebyly zachyceny a v budoucnosti by nikdo neznal dějiny lékáren v letech 1989- 2004. Jedná se o historicky velice zajímavé období. Teprve budoucí generace ocení velikost této doby, kdy se prakticky v celé České republice i v jednotlivých regionech počet lékáren...
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Influence of Economic Crisis on The Integration of European Union
Bartušková, Hana ; Karpová, Eva (advisor) ; Němcová, Ingeborg (referee) ; Křelinová, Jana (referee) ; Kučerová, Zuzana (referee)
This thesis focuses on the changes in the European Union due to the economic crisis in 2008/2009. The thesis defines an integrational system of the European Union and the changes in the system that bring some changes to the economic integration of the European Union. The main research question is whether the economic crisis brings the deepening of the economic integration or some desintegration tendencies in the European Union. The main part is the analysis of the free movement of goods, services, people and capital on the single market in the European Union. With an accent on the analysis of the institutional integration in the European Union. The thesis looks into the connection between the instutional and real integration of the European Union and the economic situation in the member states.
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Czech Television and Youth Audience
Kučerová, Zuzana ; BENDOVÁ, Helena (advisor) ; MATHÉ, Ivo (referee)
The thesis Czech Television and Youth Audience focuses on whether Czech Television as a public service broadcaster in the Czech Republic produces
enough programmes targeted at teen audience. The aim of the study is to consider if a television as a platform for watching audio-visual content is
still appealing for pubescents or whether they rather choose the Internet. Furthermore, I describe the ways by which other European public service
broadcasters attract young audience and make a suggestion to improve the situation.
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Political and social aspects of local government - public participation in the municipality affairs
Kučerová, Zuzana ; Kopřiva, Radek (advisor) ; Lenka, Lenka (referee)
The thesis deals with contemporary theories of the citizens involvement in municipal life and civic society. It works with Nekol s concept which is a typology of forms of participation. The aim of the thesis is to test the usefulness of this concept on two selected municipalities in the Czech Republic Týnec nad Labem and Krakovany.
The thesis focuses mainly of the description of participation forms and it examines the factors which influence the differences in people s participation. It shows that Nekol s concept is a useful tool in the effort to understand these differences in the two selected municipalities. The decisive distinguishing aspect is the stability of political environment. In Krakovany, the political environment is settled and is connected with the mayor, who is an important initiator of development in the town.
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Indikátory sociálního pilíře udržitelného rozvoje na lokální úrovni
Kučerová, Zita
Kvalita sociálního prostředí je nedílnou součástí kvality života jako celku. Sociální prostředí města formuje jeho tvář, naše dojmy a emoce, které na nás ve městě působí. O tom, jak měřit sociální prostředí města je vedeno nesčetné množství úvah, přístupů, postupů. Víme, co sociálním prostředí města chápeme. Sociální prostředí vytváří sociální struktura jedinců i sociální chování skupiny. Chování skupiny popisuje názory, postoje mezi skupinami a jednotlivci a je samozřejmě ovlivněno prostředím. Sociální prostředí spoluvytváří též míra komunitního života, sociální soudržnosti, role občanské společnosti a obecně zájem o věci společné. Dokážeme jej však měřit? Dokážeme stanovit, kdy je život ve městě „kvalitní“, kdy je sociální prostředí na „dobré“ úrovni? Jak a čím toto těžko hmatatelné prostředí indikujeme? Odpověď alespoň z části na tyto otázky je cílem mé disertační práce.
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Indicatours of sustainable development social pillar at the local level
Kučerová, Zita ; Kyselka, Mojmír (referee) ; Doc.PhDr.Jana Šafránková,CSc. (referee) ; Staňková, Eva (referee) ; Koutný, Jan (advisor)
The aim of the thesis is to find the answer, whether it is plausible to monitor social cohesion of the municipality and how to measure this cohesion, which is one of the essential pillars of sustainable development. In order to find out, analysis of approaches and measurements of the sustainable development social pillar in international and Czech environment was carried out. Social pillar was identified and described also at the local level. Within the framework of the sustainable development social pillar at the municipal level three main thematic blocks are proposed: • Social environment quality of the municipality • Social-demographic population structure of the municipality • Contentment and participation in the public affairs These themes should be monitored by the set of indicators proposed in this thesis. It aimed to cover all dimensions of social cohesion which are significant and well-founded in the context of the territorial planning process. These indicators can be followed in the direct relationship with the population size and regional importance of the observed territorial unit at the local level and they are not separated from the concept of indicators monitoring at other – territorially higher – levels. Outcomes, analysis and interpretation of attained values could become the basis for the “territorial sustainable development analysis” (part of territorial planning instruments according to contemporary legal regulations), representing social pillar. All phenomenon and processes must be evaluated in the context with other sustainable development pillars and afterwards they should become another suitable instrument for measurement of the fulfilling the territorial planning aims.
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Indicatours of sustainable development social pillar at the local level
Kučerová, Zita ; Kyselka, Mojmír (referee) ; Doc.PhDr.Jana Šafránková,CSc. (referee) ; Staňková, Eva (referee) ; Koutný, Jan (advisor)
The aim of the thesis is to find the answer, whether it is plausible to monitor social cohesion of the municipality and how to measure this cohesion, which is one of the essential pillars of sustainable development. In order to find out, analysis of approaches and measurements of the sustainable development social pillar in international and Czech environment was carried out. Social pillar was identified and described also at the local level. Within the framework of the sustainable development social pillar at the municipal level three main thematic blocks are proposed: • Social environment quality of the municipality • Social-demographic population structure of the municipality • Contentment and participation in the public affairs These themes should be monitored by the set of indicators proposed in this thesis. It aimed to cover all dimensions of social cohesion which are significant and well-founded in the context of the territorial planning process. These indicators can be followed in the direct relationship with the population size and regional importance of the observed territorial unit at the local level and they are not separated from the concept of indicators monitoring at other – territorially higher – levels. Outcomes, analysis and interpretation of attained values could become the basis for the “territorial sustainable development analysis” (part of territorial planning instruments according to contemporary legal regulations), representing social pillar. All phenomenon and processes must be evaluated in the context with other sustainable development pillars and afterwards they should become another suitable instrument for measurement of the fulfilling the territorial planning aims.
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Production of newspaper
Kučerová, Zuzana ; KALLISTA, Jaromír (advisor) ; Badač, Peter (referee)
The bachelor's thesis "Production of festival newspaper; comparison of Festival Daily and Film Sheets" deals with a comparison of the festival periodicals as the production. The aim of the study is to describe the conditions of production of the festival newspaper at the International Film Festival in Karlovy Vary and Summer Film School in Uherské Hradiště in 2014, and show their influence on the final form of periodicals. Furthermore, these production environments will compare, evaluate and propose a basis for improving production conditions in the coming years.
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