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Optical Fiber Element of Sensors with Biolumiscent Cells
Vrbová, Hana ; Kuncová, Gabriela ; Pospíšilová, Marie
In this work, we compared theoretical calculations [2] of coupling efficiency with experiments for various OFEs with the immobilized whole-cell bacterial bioreporter of Pseudomonas fluorescens HK44 (producing bioluminescence in presence of naphthalene and salicylate). We evaluated the influence of the physical properties and geometry of the OFE, active layer and cell immobilization techniques on intensity of detected light.
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Optické senzory pro monitorování bioprocesů
Podrazký, Ondřej ; Matějec, Vlastimil ; Kuncová, Gabriela ; Pospíšilová, Marie ; Mrázek, Jan ; Kašík, Ivan ; Rose, K. ; Jaffrezic-Renault, N. ; Dzyadevych, S. ; Šašek, L. ; Scully, P. J.
The contribution presents results of the research of an optical glucose sensor. The detection of oxygen depletion caused by glucose oxidation under glucoseoxidase catalysis was used in the glucose sensors. Fluorescence quenching of ruthenium complexes by oxygen was used for the oxygen detection. The complexes and enzyme were immobilized in inorganic-organic ORMOCER membranes. Developed fiber-optic sensors enable to detect glucose in concentrations up to 3 mmol/L and with response times of about 20s.
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