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Detached house
Macháčková, Klára ; Kučera, Radim (referee) ; Struhala, Karel (advisor)
This bachelor thesis deals with the design of a family house in Olešnice. The family house is designed as a two-storey, partially basement building, roofed by flat roof. The project was created for the purpose of designing a permanent home for a 5 persons in two housing units with the possibility of two garage parking spaces. The entrance of the house is oriented the south-eastern side to the staircase, where you can enter the basement, apartment in 1.NP or apartment in 2.NP. After entering the apartment in 1.NP the person is located in vestibule from which you will get into the kitchen with a living room, room, bedroom, bathroom, toilet, dressing room or storage room. The apartment in 2.NP is living room, bedroom, kitchen with living room, two bedrooms, bathroom, toilet, cloakroom and balcony. In basement are three storage areas, a workshop and a technical room. Social and private zones are oriented mostly on the southwest side. The perimeter structures are in the basement from lost formwork BEST which is insulated with extruded polystyrene. The rest of the vertical structures are made of ceramic blocks Porotherm. The ceiling structure is solved by the Porotherm system. Part of the proposal is thermal engineering assessment, fire safety solutions and assessment in terms of acoustics and daylight. The thesis contains a drawings and technical documentation and it is composed as an implementation project.
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Apartment building
Duben, Tomáš ; Kučera, Radim (referee) ; Struhala, Karel (advisor)
The main topic of this bachelor thesis is design of a new apartment building in Havlíčkův Brod. It is designed as a two-floor building with partial basement. The apartment building contains four separate apartments each of which is meant for a four member family. There is a partly walkable vegetation roof and the construction of the second floor above the first floor. Access to the walkable roof from the apartments in the second floor is available. The vertical supporting structures are ceramic Porotherm system and shuttering system filled with concrete C20/25 and reinforcement steel B500B. Ceiling structures are made from prestressed floor panels Spiroll. The outside parking is included on the building plot. Both the designs and visualization were elaborated in the ArchiCad software. The designs meet the valid standards and regulations.
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LC-HRMS analysis of selected antihypertensive drugs in biological material for compliance assessment
Tláskalová, Anna ; Kučera, Radim (advisor) ; Štěrbová, Petra (referee)
5 ABSTRACT Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Anna Tláskalová Supervisor - specialist: Mgr. Martin Mžik Supervisor: doc. PharmDr. Radim Kučera, Ph.D. Title: LC-HRMS analysis of selected antihypertensive drugs in biological material for compliance assessment About 40 % of Czech population between the ages of 25 and 64 suffer from arterial hypertension. Although an array of effective antihypertensives is available nowadays, optimal blood pressure during treatment is reached by mere 30 % of the patients. This is mainly due to the patients' poor adherence to the treatment, who use their medicaments incorrectly or not at all. The adherence of a patient to the treatment, as well as pharmacokinetics, represent the most significant source of variability of the answer to the treatment and notably influences its outcome. The monitoring of plasmatic levels of antihypertensives is one of the methods of modern medicine which enables both an effective supervision of the incorrectly compensated patients, and the adjustment of dosage scheme. This diploma thesis focuses on development and optimisation of extraction procedures for selected antihypertensives (amiloride, amlodipine, betaxolol, bisoprolol, carvedilol, celiprolol,...
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GC analysis of drugs with utilization of ionic liquid as a stationary phase I
Bärová, Karolína ; Kastner, Petr (advisor) ; Kučera, Radim (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Supervisor: PharmDr. Petr Kastner, Ph.D. Student: Karolína Bärová Title of Thesis: GC analysis of drugs with utilization of ionic liquid as a stationary phase I In this thesis, we focused on testing of three commercially obtainable columns with ionic liquids as stationary phases, which we tested on a model sample of ibuprofen and its four pharmacopoeial impurities after derivatization by alkylchloroformates. The results obtained on these columns were compared to results obtained on column with conventional nonpolar stationary phase. Furthermore, we tried to describe and explain retention mechanisms, which were important in these separations, we also tried to optimize methods for our individual columns and extract our derivatives into a nonpolar phase, with the view to diminishing the harmful effect of samples on columns. We appraise the results obtained on ionic liquid stationary phases and on a nonpolar stationary phase as quite comparable. The analysis of our derived samples can be performed on all tested ionic liquid columns and the analysis can be optimized for all of them. Apart from the change in selectivity on the most polar stationary phase, we did not notice any great...
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Non-silica based materials in drug analysis IV.
Ženíšková, Tereza ; Kučera, Radim (advisor) ; Pilařová, Pavla (referee)
Non-silica based materials in drug analysis IV. Diploma thesis Tereza Ženíšková Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Heyrovského 1203, Hradec Králové. Sample preparation is an important step before the analysis itself. It enables removal of the ballast components, which could interfere with an analyte or even disable the analysis. Solid phase extraction (SPE) belongs among the most popular sample preparation techniques. Silica is the most often used sorbent for SPE, however new, more durable sorbents with different selectivity have appeared. This thesis focuses on employment of zirconium dioxide and its ability to retain analytes having Lewis base character via ligand exchange. We tested to use zirconium dioxide in extraction of indomethacin as a Lewis base from suppositories. The influence of conditioning solvents with different polarity and extractions solvents was investigated. 10% acetonitrile in methylene chloride was found to be the best solvent for conditioning of zirconia. Methanolic ammonia solution was used as eluent. After some experiments the concentration and the volume of the eluent was set at 0.25M NH3 in MeOH and 3 mL. Diclofenac was used as the internal standard. The recovery for sorbent after...
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Anthelmintic and other xenobiotic biotransformation in helminths and its contribution to resistance development
Prchal, Lukáš ; Szotáková, Barbora (advisor) ; Hrdý, Ivan (referee) ; Kučera, Radim (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate Mgr. Lukáš Prchal Supervisor prof. Ing. Barbora Szotáková, Ph.D. Title of Doctoral Thesis Anthelmintic and other xenobiotic biotransformation in helminths and its contribution to resistance development Parasitic helminths such as tapeworms, flukes or nematodes pose a threat for domestic, farm and wild living animals. Helminths cause significant health problems in animals. Moreover, they cause economical losses to farmers due to production decrease and treatment costs. The treatment with veterinary anthelmintics is still a basic method to fight off helminth infections. However, the long-term use of anthelmintics caused an emergence of resistance to anthelmintics. The increasing incidence of multiresistant strains of many helminth species is the greatest problem. Therefore, research of drug resistance mechanisms became an actual field of study. Increased biotransformation of anthelmintics, transport out of parasite bodies and contact of lower developmental stages with anthelmintics in the environment are some of possible mechanisms that lead to decrease of anthelmintic therapy effectivity and resistance development. The presented thesis focuses on the study of these mechanisms in three helminth...
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Synthesis of new organic compounds containing chalcogens
Miltová, Lucie ; Kučera, Radim (advisor) ; Kučerová, Marta (referee)
Charles University, Faculty of Pharmacy in Hradec Kralove Department of Pharmaceutical Chemistry and Drug Analysis Candidate Lucie Miltová Supervisor doc. PharmDr. Radim Kučera, Ph.D. Consultant Prof. Dr. Claus Jacob Title of Diploma Thesis Synthesis of new organic compounds containing chalcogens A new triazole containing selenium was prepared by Huisgen 1,3-dipolar cycloaddition of alkyne and azide, known as the click reaction. Another two tetrazoles were synthesized by click reaction. Both compounds have the same structure, except the first compound is tetrazole bound to selenium, in the second compound to sulfur. Additionally, two more compounds contain the SeCN fragment and sulfur at the aromatic ring was prepared. The focus of this thesis is on the synthesis of new organic compounds containing chalcogens, specifically sulfur and selenium. Due to the presence of chalcogens in the structure, they are expected to show antioxidant, anticancer, antifungal or antibacterial effects or a combination thereof. A new triazole was synthesized and subsequently characterised by TLC, 1 H-NMR, 13 C- NMR and MS. It is suitable for future testing of biological activity. Tetrazole contains sulfur was prepared. The final product is, however, unstable and prone to rapid degradation. Using the same reaction...
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The employment of HPLC in the field of chiral separations III.
Janetková, Monika ; Kučera, Radim (advisor) ; Nobilis, Milan (referee)
Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy in Hradec Králové, Charles University Candidate: Monika Janetková Supervisor: doc. PharmDr. Radim Kučera, Ph.D. Consultant: doc. Ing. Radim Vespalec, DrSc. Title of Thesis: The employment of HPLC in the field of chiral separations III. Boron cluster compounds are completely synthetic inorganic structures posessing unique properties. Exceptional characteristics allow them to prove competent among organic molecules predominantely used in medicine. Basic structure is made of boron and hydrogen atoms forming three-dimensional polyhedron with triangular planes. The atoms B-H-B are forming electrondeficient three-centred two-electron bond. Cloud of electrons that is shared inside polyhedron structure is the cause of delocalized negative charge of a whole molecule. Boron cluster compounds are likely to loose hydrogen proton in both, protic and aprotic solvents, ending in formation of an anion. Among their typical characteristics belong extreme hydrophobicity, metabolic and thermal stability. Considering stability and reactivity closo-, nido- and arachno-clusters with 11-12 vertices are largely used. Either of endo- or exoskeletal substitution might impair symmetry of a boron cluster molecule resulting in a formation of...
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Semipreparative separation of bioconjugates of azaphtalocyanines
Novotná, Marta ; Kučera, Radim (advisor) ; Pilařová, Pavla (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical analysis Candidate: Mgr. Marta Novotná Consultant: doc.PharmDr. Radim Kučera, Ph.D. Title of thesis: Semipreparative separation of bioconjugates of azaphtalocyanines This rigorous thesis deals with the development of suitable chromatographic conditions for the semipreparative separation of bio-conjugates of azaphthalocyanines. These compounds are of great interest today, thanks to their employment as potential quenchers of fluorescence in the molecular probes. The experiments were carried out in analytical and semi-preparative scales. The conditions of separation in the analytical as well as semi-preparative scale were optimized by changing the mobile phase composition. The following chromatographic conditions were found for separation of bio-conjugates of azaphtalocyanines: Stationary phase: Hypersil BDS®, 150 × 10 mm, 5 μm particle size Mobile phase A: 50 mM water solution TEAA in 12 % acetonitrile Mobile phase B: acetonitrile Isocratic elution A : B 57 : 43 (v/v) Other conditions: temperature 40 řC, flow rate 2,5 ml per minute Detection: 254 nm, 673 nm The desired bio-conjugate of azaphtalocynine were successfully isolated from the reaction mixture using the developed...
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Modification of the capillary wall for the separation purpose I.
Blažková, Andrea ; Kučera, Radim (advisor) ; Kastner, Petr (referee)
Department of Pharmaceutical Chemistry and Drug Analysis Andrea Opavská doc. PharmDr. Radim Kučera, Ph.D. Modification of the capillary wall for the separation purpose I In general, capillary electrophoresis is a separation method suitable for the analysis of charged molecules, eventually neutral substances. The interest in this method and its further modification (e.g. capillary electrochromatography) has been increasing in recent years, is developing. In the case of capillary electrochromatography, the development focuses mainly on the modification of the inner wall of the capillary. The purpose of this modification is in particular to introduce a stationary phase that would improve selectivity, as well as the modification of the electroosmotic flow. The thesis deals with the modification of the internal capillary wall and the subsequent testing of the prepared capillary. One possibility is chemical coating. The second tested method is called Layer-by-Layer and represents the layering of variously charged substances in layers interacting with each other through electrostatic forces. In our work we used polycation poly(diallyldimethylammonium chloride) and graphene dispersion. The choice of graphene is very advantageous because it has excellent adsorption properties due to its morphological...
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