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National Repository of Grey Literature

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Soluble endoglin effects on endothelial dysfunction markers in mice Bountalis, Konstantinos - Spyridon ; Nachtigal, Petr (advisor) ; Prašnická, Alena (referee) Charles University Department of Biological and Medical Sciences Candidate: Konstantinos Bountalis Supervisor: prof. PharmDr. Petr Nachtigal, Ph.D. Title of diploma thesis: Soluble endoglin effects on endothelial dysfunction markers in mice Endothelial dysfunction is associated with modification of endothelial cells amount failing to maintain vascular homeostasis and result in variable pathological inflammatory states as well as in progression of vascular damage. Particularly its state is characterized by high expression of cell adhesion molecules, reduction of vasodilation and accumulation of cholesterol molecules. The purpose of this study is to detect soluble endoglin effects on the expression of VCAM-1 and ICAM-1 in aorta in mice. The experiment was accomplished utilizing specific immunohistochemical methods for the detection of expression of selected adhesion molecules. High fat diet administration resulted in development of mild hypercholesterolemia in mice in both groups. High levels of soluble endoglin however did not significantly affect the expression of VCAM-1 and ICAM-1 in aortic endothelium when compared to mice with low levels of soluble endoglin. The results of this study did not show pro-inflammatory effects of soluble endoglin in aorta in mice. Detailed record

Diagnostics of exanthematic childhood diseases Slámová, Zuzana ; Doleželová, Eva (advisor) ; Prašnická, Alena (referee) Zuzana Slámová Diagnostics of exanthematic childhood diseases Bachelor thesis Charles University, Faculty of Pharmacy in Hradec Králové Health bioanalytics Background: The aim of the bachelor thesis was to determine the morbidity, incidence and prevalence of selected exanthematic childhood diseases (measles, scarlet fever, rubella, fourth disease, fifth disease and chickenpox) in the period 2013 - 2016 at the Department of Clinical Microbiology and Immunology of Regional Hospital Liberec, a. s. including a comparison with occurance of diseases in the whole Czech Republic. Methods: The methodical part was focused on the methods which are the basis of specific analyzers at the Department of Clinical Microbiology and Immunology of the Regional Hospital Liberec, a. s. These methods are ELISA, CLIA, KFR, PCR and cultivation. Analyzers used to diagnose antibodies against selected diseases are Alegria® (ORGENTEC), LIAISON® XL (DiaSorin) and Chorus Trio (DIESSE). For the direct quick detection of the disease was used quantitative PCR (qPCR) method on the CFX96 Touch ™ Real-Time PCR Detection System (BioRad). Detection of bacterial agents was performed by cultivation. Results: The results were processed over the period 2013 - 2016 from data provided by the Department of Clinical Microbiology and Immunology... Detailed record

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