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Effect of selected abiotic additives on bioremediation of crude oil contamination in soil
Kuráň, P. ; Trögl, J. ; Nováková, J. ; Pilařová, V. ; Pilařová, K. ; Ederer, J. ; Dáňová, P. ; Pavlorková, J. ; Fryčák, R. ; Bošková, S. ; Kozler, J. ; Novák, František
The effect of addition of selected abiotic additives (humic substances, zeolites) on the biodegradation of crude oil in polluted soil by joint action of autochthonic micro flora as well as by crude-oil-degrading bacterial strain was tested in the set of pot experiments. The process was followed by means of analytical parameters (hydrocarbon index, ratios pristane/C.sub.17./sub., phytane/C.sub.18./sub., total organic carbon content) and characterization of activity and structure of microbial community within one-year-experiment. Addition of humic substances did not affect biodegradation rate significantly, however it improved the evolution of soil microbial community biomass. Addition of zeolites (10% w/w) resulted in slower increase of microbial biomass compared to modules lacking zeolites, however it improved the operation with soil. In contrast, augmentation of degrading bacterial strain increased the biodegradation rate significantly and proved to be the most effective treatment.
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