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National Repository of Grey Literature

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Effect of hypoxia on embryonic development of the heart and coronary circulation Křížová, Petra ; Naňka, Ondřej (advisor) ; Sedmera, David (referee) Human, as all multicelullar organisms, is dependant on oxygen availability. Hypoxia is a stimulus which must the organism adapt from early development. In prenatal life fundaments of circulation originate separately and first than they interconnect in complete system of vessel tubes incorporating heart. Haemodynamic - as one of the factors - soon takes part in vessels system development. Restricted blood flow results in vascular bed regression; on the contrary higher flow promotes its development in prenatal life as well as in adulthood. The aim of our study was to investigate the effect of hypoxia on coronary arterial tree formation and myocardial development and to explain embryonic lethality. Previous studies found ventricular dilatation and increased mortality with signs of heart failure. We used quail embryos (Coturnix coturnix japonica). Eggs were incubated under hypoxic conditions (16% O2). Controls were incubated in normal oxygen tension (21% O2). The effect of hypoxia was analyzed 5 - 9 day of embryo development. Coronary system was visualized by injecting Indian ink. Proliferation was measured using BrdU labelling. For histological analysis we used standard haematoxylin and eosin labelling. For further analysis we used immunohistochemical staining with antibodies against sarcomeric actin... Detailed record

Miniinvazivní chirurgie primární hyperparathyreózy Adámek, Svatopluk ; Naňka, Ondřej ; Univerzita Karlova. Detailed record

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