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Study of interaction in biopolymer-surfactant system
Sovová, Šárka ; Mondek, Jakub (referee) ; Pilgrová, Tereza (advisor)
Interaction between biopolymer-surfactant and stability of arising complexes were investigated using dynamic light scattering method Stability of the system and interactions between native hyaluronan and cationic surfactant cetyltromethyl-amonium bromide in aqueous solution depending on preparation method were investigated. System was prepared either adding CTAB to hyaluronan causing sequential micelle’s formation, or adding hyaluronan to already formed micelles. It was found out that preparing method influences properties of arisen complexes. Molecular weight of hyaluronan has an inconsiderable effect on interaction against to concentration of hyaluronan, which influences interaction of component significantly.
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Fluorescence correlation spectroscopy in hydrophilic colloids
Rýcová, Eva ; Mondek, Jakub (referee) ; Venerová, Tereza (advisor)
This work is focused on studying of hydrophilic colloids using fluorescence correlation spectroscopy (FCS). The gel systems were studied with several fluorescent probes, in 0,15 M sodium chloride (NaCl) consist of hyaluronan and surfactant cetyltrimethylammonium bromide (CTAB). Probe ATTO 655 was chosen for closer examination due to the suppression of triplet state. Every surrounding occuring in the gel systems was examine individually. Results showed that the measurement of gel systems by this method can be realized under certain conditions, the repeatability of the results was loaded with a small standard deviation. Extension of diffusion times caused the presence of micelles, triplet state was caused by a characteristic environment of the hydrogel. The probe also showed free diffusion motion in all environments. This diffusion motion was represented by the shortest diffusion time.
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Microrheology with Fluorescence Correlation Spectroscopy
Kábrtová, Petra ; Sedláček, Petr (referee) ; Mondek, Jakub (advisor)
A comparison of three passive microrheological techniques was made with the emphasis on FCS. Fluorescently labelled and unlabelled polystyrene particles were used to probe a microrheological response of glycerol solutions, Mili-Q water and sodium hyaluronate solutions. In addition, for FCS technique an approximated equation for calculation of MSD values was derived and verified. It was found that FCS outmatches current microrheological techniques of DLS and video-based particle tracking by its ability to gain a broader data range including the area in which, until now, it was impossible to describe a microrheological behaviour of samples reliably.
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Cationic fluorescence probes in a polyanion-surfactant system.
Mondek, Jakub ; Šmejkalová, Daniela (referee) ; Pekař, Miloslav (advisor)
In this bachelor thesis were studied properties of aqueous solutions of hyaluronan and celytrimethylamonnium bromid with help of acridine orange as fluorescence probe. At first, this thesis was focused on interactions of acridine orange and hyaluronan. Emision and excitation spectra of acridine orange were observed. Result of these measurement was bonding of acridine orange dimer to carboxylic group of hyaluronan. Next studied interaction was interaction of cetyltrimethyl amonium bromid with acridine orange – hyaluronan system. Result of these interaction was, that molecules of cationactive surfactant pushed away dimers of acridine orange and took place of dimer of acridine orange on carboxylic group of hyaluronan. Dimer of acridine orange was changed into monomers. The reason why this thesis was focused on study of aqueus solutions of hyaluronan with cationactive surfactant is benefit of hyaluronan – surfactant system in research of transport system for target distribution of drugs.
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