National Repository of Grey Literature 12 records found  previous11 - 12  jump to record: Search took 0.00 seconds. 
Purification of caseinomacropeptide by novel solution phase isoelectric focusing device
Duša, Filip ; Šlais, Karel
We presented a new solution phase isoelectric focusing device based on nonwoven strip devised in our laboratory. This device was used for purification of caseinomacropeptide from crude whey.
Using of divergent flow isoelectric focusing and capillary liquid
Duša, Filip ; Moravcová, Dana ; Kahle, Vladislav ; Šlais, Karel
Separation of analytes from complex matrices play important role in their further identification. In this article we propose two-dimensional separation method of bovine serum albumin tryptic peptides. At the first dimension peptides are separated by divergent flow isoelectric focusing followed by reverse-phase liquid chromatography as the second dimension. Due to composition of input mixture it is possible to use autofocusing mode in first dimension. In this mode peptides are focused without addition of carrier ampholytes. Chromatograms from the second dimension were processed into contour map. Acquired data showed good separation efficiency of isoelectric focusing. Autofocusing mode enabled better resolution of peptides with close isoelectric points.

National Repository of Grey Literature : 12 records found   previous11 - 12  jump to record:
Interested in being notified about new results for this query?
Subscribe to the RSS feed.