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The importance of extracurricular education in Geography on the 2nd grade of primary school (using the example of activities of the Pionyr organization)
BENDOVÁ, Petra
The bachelor theses deals with the extracurricular education of geography on the 2nd grade of the primary school with emphasis on activities of the Pionyr organization. The main goal of this thesis is to find the meaning of the extracurricular education of Geography and creating a few practical activities focused on the Pionyr organization. The theoretical part deals with the Framework Education Programme for Primary Education, the extracurricular education and the Pionyr organization. The practical part includes the summary of events that took place during 2011-2016 under the Pionyr group Kamarad from Jihlava. The events that have geographical aspects are then analysed with respect to their contents, classification in the Framework Education Programme for Primary Education in Czech Republic, and cross-curricular subjects. The author created a few new activities and extended some of the past activities on the basis of this analysis. These activities can be later used during the events of the Pionyr organisation.
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Analysis of chromosomal aberrations in sperm by fluorescence in situ hybridization
Bendová, Petra ; Diblík, Jan (advisor) ; Novotná, Drahuše (referee)
The presented bachelor work is focused on the determination of frequency chromosomally abnormal sperm in the semen of healthy men (donors) with normal karyotype (46, XY). The important process, which plays an irreplaceable role in the development of numerical aberrations of chromosomes or structural abnormalities in the segregation of the gametes, is meiosis. Therefore, I devote much attention on meiosis in the theoretical part. The theoretical part is focused on the process of pre mature sperm (spermatogenesis), and the consequences of fertilize the oocyte by aneuploid sperm. In my work I present an overview of numerical abnormalities in autosomes and gonosomes and their frequency and distribution of gametes in healthy men. I also focused on the distribution and a brief description of structural aberrations affecting chromosomes and not least I paid attention on method of multicolor interphase fluorescence in situ hybridization, which in combination with sperm chromatin dekondenzation become irreplaceable and valuable research tool for rapid analysis of chromosomal abnormalities in large sperm samples. The experimental part of bachelor work deals with monitoring the frequency of selected numerical abnormalities in sperm samples of five donors aged 23 to 30 years with the use of I-FISH (fluorescence in situ...
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Study of new iron chelators for the prevention of oxidative stress-induced cardiomyocyte injury
Bendová, Petra ; Šimůnek, Tomáš (advisor) ; Kvasničková, Eva (referee)
Results of many studies demonstrate a significant role of oxidative stress in the pathogenesis of many illnesses including cardiovascular diseases. Free cellular iron plays significant role in development of oxidative cell damage; it catalyzes formation of hydroxyl radicals. Hydroxyl radicals belong among the most reactive and toxic forms of reactive oxygen species (ROS). Hence, iron chelators can be used as preventive agents against the development of oxidative damage of tissues. This study deals with confirmation of ability of the iron chelator salicylaldehyde isonicotinoyl hydrazone (SIH) to prevent this damage and with the study of properties of new aroylhydrazone chelators derived from SIH. Protective effects against a model oxidative stress and own toxicities of individual agents were evaluated in vitro using rat cardiomyoblast cell line H9c2, the viability of cells was determined by neutral red uptake assay. A 24-hour incubation of H9c2 cells with 200 µM tert-buthyl hydroperoxide (t-BHP) evoked dramatic changes of cellular morphology and was followed by cell death. Co- incubation with all chelators dose-dependently reduced or abolished cell damage. The replacement of salicyl aldehyde in SIH with a methyl ketone led to an amplification of protective properties of this ligand, this chelator is...
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Study of new iron chelators for the prevention of oxidative stress-induced cardiomyocyte injury
Bendová, Petra ; Kvasničková, Eva (referee) ; Šimůnek, Tomáš (advisor)
Results of many studies demonstrate a significant role of oxidative stress in the pathogenesis of many illnesses including cardiovascular diseases. Free cellular iron plays significant role in development of oxidative cell damage; it catalyzes formation of hydroxyl radicals. Hydroxyl radicals belong among the most reactive and toxic forms of reactive oxygen species (ROS). Hence, iron chelators can be used as preventive agents against the development of oxidative damage of tissues. This study deals with confirmation of ability of the iron chelator salicylaldehyde isonicotinoyl hydrazone (SIH) to prevent this damage and with the study of properties of new aroylhydrazone chelators derived from SIH. Protective effects against a model oxidative stress and own toxicities of individual agents were evaluated in vitro using rat cardiomyoblast cell line H9c2, the viability of cells was determined by neutral red uptake assay. A 24-hour incubation of H9c2 cells with 200 µM tert-buthyl hydroperoxide (t-BHP) evoked dramatic changes of cellular morphology and was followed by cell death. Co- incubation with all chelators dose-dependently reduced or abolished cell damage. The replacement of salicyl aldehyde in SIH with a methyl ketone led to an amplification of protective properties of this ligand, this chelator is...
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Alternative and augmentative communication at children with cerebral palsy
Bendová, Petra
Magdalény Rettigové 4, 116 39 Praha 1 tel.: +420 221 900 111 www.pedf.cuni.cz IČ 00216208 DIČ CZ00216208 Dle čl. 4 Opatření rektora č. 6/2010 o Zpřístupnění elektronické databáze závěrečných prací http://www.cuni.cz/UK-3470.html a čl. 1 Opatření děkana 17/2010 se z časového hlediska závěrečné práce dělí do tří skupin: a. "nové práce", tj. práce odevzdávané k obhajobě počínaje 29. 9. 2010, b. "starší práce", tj. práce odevzdané k obhajobě od 1. 1. 2006 do 28. 9. 2010, c. "práce před rokem 2006", tj. práce odevzdané k obhajobě před 1. 1. 2006. V tomto případě jde o "starší práci" odevzdanou k obhajobě od 1. 1. 2006 do 28. 9. 2010. Omlouváme se, ale dokument není k dispozici.
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