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Injury to the distal tibiofibular junction in the growing skeleton
Pešl, Tomáš ; Bartoníček, Jan (advisor) ; Koudela, Karel (referee) ; Krbec, Martin (referee)
In adults the complex fractures of the ankle joint have been thoroughly discussed and there are several therapeutic schedules proposed. Weber distinguished three types of ankle joint fractures according to the type of fibular fractures. In agreement with this classification we are certain that fibula is an important element of the immature ankle joint. We concentrated on the anatomy of immature ankle joint, especially on the mutual position of the distal fibular physis and the plane of the tibiotalar joint. The results are summarized as follows: 9.1. AUTOPSY At first we performed autopsy of six ankle joints in stillborns in which there were no orthopaedic and/or neurological lesions. We paid attention to anatomy of the distal tibiofibular junction. Two adult ankles were anatomised for correlation the findings. We ascertained, that in all preparations there was articulation between tibia and fibula, as it is formerly described in the literature. In all six stillborn joints we found well formed ligaments of the distal tibiofibular junction: anterior, posterior and interosseal tibiofibular ligaments. The anterior and posterior tibiofibular ligaments begin at the anterior, respectively posterior surface of the distal tibial epiphysis and terminate at the anterior, respectively posterior surface of the distal...
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Device for wavefront measurement of microscope objective lenses
Bartoníček, Jan ; Šerý, Mojmír (referee) ; Slabý, Tomáš (advisor)
The wavefront reconstruction of a light wave transformed by a microscope objective is the main subject of this diploma thesis together with the design and assembly of a~measuring device and the development of a computational algorithm. The purpose is to determine optical aberrations and to compare a quality of objectives with identical parameters. The term wavefront is explained and its description using Zernike polynomials is introduced in the first part of the thesis. The following part summarizes possible methods for wavefront reconstrucion. Two methods were chosen for experimental determination of a wavefront shape – shearing interferometry and solution of the transport of intensity equation. For each method a brief characteristic is provided together with possible applications, mathematical apparatus, image processing, computational procedure, setup description and proposition and results of experiments. The suitability of both methods for optical aberration determination and microscope objective comparison is discussed. Based on the obtained results, both methods were found to be suitable for comparison of microscope objectives. The suitability for optical aberration determination is possible with certain restrictions.
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Coherence-controlled holographic microscope in cell's life cycle research
Bartoníček, Jan ; Chmelík, Radim (referee) ; Uhlířová, Hana (advisor)
The subject of the bachelor thesis is live-cell imaging in a transmitted-light holographic microscope which was designed at the Institute of Physical Engineering BUT and comparing this imaging method with the phase-contrast microscopy. The first part is dedicated to a basic description of used imaging techniques and a cell biology. A description of an experiment preparation follows. In the part dedicated to a data analysis the method of dynamic phase differences is described and the method of growth monitoring is proposed. Both methods were used for the analysis of experiments which are described in the last part of this work. Experiments were focused on acquiring time-lapse data of a cell’s cycle and particularly the mitosis.
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