|
|
Synthesis and study of hyaluronic acid derivatives modified on carboxyl
Volfová, Gabriela ; Roh, Jaroslav (advisor) ; Opálka, Lukáš (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Organic and Bioorganic Chemistry Author: Gabriela Volfová Supervisors: Mgr. Radovan Bu a, Ph.D. doc. PharmDr. Jaroslav Roh, Ph.D. Title: Synthesis and study of hyaluronic acid derivatives modified on carboxyl Abstract: Hyaluronic acid (HA) is a naturally occurring linear polysaccharide. Due to its biocompatibility and non-immunogenicity, HA and its derivatives are widely used in various biological and medical applications. The e cacy of each formulation is strongly related to the molecular weight (MW) of the HA used. Unfortunately, various factors can a ect the MW during the manufacturing process. Elevated tem- perature required during the sterilization process is one significant example, which can lead to a reduction in molecular weight and result in a loss of some desired properties of the final product. This work focuses on the synthesis of HA amides formed on its free carboxyl group and subsequently studying the e ect of this modifi- cation on the degradation rate at elevated temperature. Three final compounds were prepared by reacting HA with various amines using 4-(4,6-dimethoxy-1,3,5-triazin-2- yl)-4-methylmorpholinium chloride (DMTMM), all of which underwent degradation noticeably faster than native HA.
|
|
|
Synthesis and study of hyaluronic acid derivatives modified on carboxyl
Volfová, Gabriela ; Roh, Jaroslav (advisor) ; Opálka, Lukáš (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Organic and Bioorganic Chemistry Author: Gabriela Volfová Supervisors: Mgr. Radovan Bu a, Ph.D. doc. PharmDr. Jaroslav Roh, Ph.D. Title: Synthesis and study of hyaluronic acid derivatives modified on carboxyl Abstract: Hyaluronic acid (HA) is a naturally occurring linear polysaccharide. Due to its biocompatibility and non-immunogenicity, HA and its derivatives are widely used in various biological and medical applications. The e cacy of each formulation is strongly related to the molecular weight (MW) of the HA used. Unfortunately, various factors can a ect the MW during the manufacturing process. Elevated tem- perature required during the sterilization process is one significant example, which can lead to a reduction in molecular weight and result in a loss of some desired properties of the final product. This work focuses on the synthesis of HA amides formed on its free carboxyl group and subsequently studying the e ect of this modifi- cation on the degradation rate at elevated temperature. Three final compounds were prepared by reacting HA with various amines using 4-(4,6-dimethoxy-1,3,5-triazin-2- yl)-4-methylmorpholinium chloride (DMTMM), all of which underwent degradation noticeably faster than native HA.
|
|
|
Udržitelný cestovní ruch v území Vývěrů Punkvy CHKO Moravský kras
Volfová, Gabriela
This thesis deals with the issue of sustainability of tourism in the bubble out area of river Punkva, which is part of the important Protected Landscape Area of the Moravian Karst. Generally there are defined concepts as tourism, environmental impacts, sustainable development. There was created an analysis of the own and foreign data. Visit of the area of interest was managed and interviews with the people connected to the locality were made. The work reveals the activities and tourism trends, which are implicated in the three pillars of sustainability. In form of the matrix of strengths, weaknesses, opportunities and threats, there was created summation of the investigated issues. The thesis concludes recommendations to correct the deficiencies that were identified by reconnaissance.
|
guest ::
