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National Repository of Grey Literature

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Vyhledávání promotorů Corynebacterium glutamicum různě regulovaných v různých fázích růstu Phensaijai, M. ; Knoppová, Monika ; Pátek, Miroslav ; Nešvera, Jan Promoters of the amino acid-producing bacterium Corynebacterium glutamicum regulated differently in various phases of growth have been searched for. It was found that activity of promoter of the sigE gene coding for alternative sigma factor of RNA polymerase was strongly increased when the culture entered the stationary phase of growth Detailed record

Modulace genové exprese mutagenezí v promotorech Corynebacterium glutamicum Holátko, Jiří ; Elišáková, Veronika ; Prouza, Marek ; Phensaijai, M. ; Nešvera, Jan ; Pátek, Miroslav Studies of structure and function of promoters in C. glutamicum were used in graded expression of genes involved in biosynthetic pathway of valine (ilvBNC, ilvD, ilvE) and in competing or branching-off pathways (ilvA, leuA). Combination of particular promoter mutants resulted in tuning the whole pathway to overall higher flux of the intermediates through the pathway and in high production of valine Detailed record

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