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Study of the microRNAs in patients with head and neck squamous cell carcinoma
Hudcová, Kristýna ; Šulc, Miroslav (referee)
Searching for markers of disease is at the forefront of scientific interest. Biomarker of a specific disease should help in disease management - whether for diagnosing a disease or predicting its progress. We picked microRNAs - short RNAs that regulate mRNA translation, due to their stability and function in the regulation of many cellular processes. Therefore they can be used as a biomarker for many diseases. In our study we focused on neck squamous cell carcinoma (HNSCC), since this type of cancer is widespread and clinically important markers are missing. It is in our interest to seek new kinds of markers, suitable for early diagnosis of the disease and the most accurate assessment of prognosis. We selected 3 candidate miRNA (miR-29c-3p, miR-200b-5p, miR-375-3p) and used qRT-PCR method to profile their expression in tissues of patients with HNSCC. MiRNA expressions in 42 tumor samples were compared with the miRNA expression in adjacent tissue of the tumor. Adjacent tissue surrounds the tumor and has interesting properties, as it looks like a histological normal tissue, but on closer inspection it is not. It is affected by tumor tissue and may contribute to tumor progression. We managed to distinguish between these two tissues using miR-29c-3p and miR-375-3p. Additional analyzes were focused on...
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Study of the microRNAs in patients with head and neck squamous cell carcinoma
Hudcová, Kristýna ; Šulc, Miroslav (referee)
Searching for markers of disease is at the forefront of scientific interest. Biomarker of a specific disease should help in disease management - whether for diagnosing a disease or predicting its progress. We picked microRNAs - short RNAs that regulate mRNA translation, due to their stability and function in the regulation of many cellular processes. Therefore they can be used as a biomarker for many diseases. In our study we focused on neck squamous cell carcinoma (HNSCC), since this type of cancer is widespread and clinically important markers are missing. It is in our interest to seek new kinds of markers, suitable for early diagnosis of the disease and the most accurate assessment of prognosis. We selected 3 candidate miRNA (miR-29c-3p, miR-200b-5p, miR-375-3p) and used qRT-PCR method to profile their expression in tissues of patients with HNSCC. MiRNA expressions in 42 tumor samples were compared with the miRNA expression in adjacent tissue of the tumor. Adjacent tissue surrounds the tumor and has interesting properties, as it looks like a histological normal tissue, but on closer inspection it is not. It is affected by tumor tissue and may contribute to tumor progression. We managed to distinguish between these two tissues using miR-29c-3p and miR-375-3p. Additional analyzes were focused on...
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Active employment policy in Pardubice region
Hudcová, Kristýna ; Adámek, Petr (advisor) ; Dobrylovský, Jiří (referee)
The thesis deals with the topic of unemployment, studying the solution lying in the active employment policy. The theoretical part defines the term labour market and unemployment together with its measurement and types. It also outlines the unemployment policy, distinguishing the passive and the active form. The practical part is focused on the Pardubice Region, describing its demographic and economic characteristics. It describes the actual situation on the regional labour market, primarily focusing on the active employment policy in the region, tools being brought to bear and the methods of funding.
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