National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Structure and function of the invasion apparatus of microsporidia
Dohnálek, Vít ; Doležal, Pavel (advisor) ; Vávra, Jiří (referee)
Microsporidia are obligate intracellular parasites that cause significant economic damage and can infect humans. They had evolved highly specialized invasion apparatus that is unique to them. During the invasion the sporoplasm is released from the spore wall and it is transferred to the host cytoplasm through the polar tube. Current research on the invasion apparatus has been focused mainly on the polar tube that is its most prominent structure. Polaroplast and posterior vacuole remain out of the main interest, although they are necessary for the activation and execution of the invasion. If the right combination of environmental factors occurs, the organization of the polaroplast begins to change, the posterior vacuole starts to swell and the polar tube is discharged. Sporoplasm is eventually pushed through the tube into the host cell by growing posterior vacuole. The mechanism has not been explained yet however plenty of theories are trying to explain the germination. This work summarizes current theories and knowledge of structure and function of particular parts of the invasion apparatus. Key words: Microsporidia, invasion, polar tube, intracellular parasitism, germination
Evolution of mitochondrial protein import
Dohnálek, Vít ; Doležal, Pavel (advisor) ; Novotný, Marian (referee)
1 Abstract Even though mitochondria possess their own genome and ribosomes, majority of mitochondrial proteins is encoded in the nucleus and translated by cytosolic ribosomes. Hence it was necessary to establish transport complexes allowing the import of proteins from the cytosol. These complexes are best described in yeast. However, we are encountering organisms lacking many of the subunits of these complexes with increasing frequency. Therefore, we are presenting the overview of the distribution of the subunits within eukaryotic organisms. We specifically take a closer look at parasitic protist Giardia intestinalis that is well known for its extreme reductions of the import complexes. There have been only few subunits identified so far. Porin Tom40, that is responsible for translocating all the incoming proteins across the outer mitochondrial membrane, has been identified despite the high divergence, while homolog of Sam50 hasn't been successfully identified yet. Sam50 is however believed to be necessary for insertion of Tom40 into the membrane. Vast part of this thesis is dedicated to this phenomenon that is highly uncommon and maybe unique among the eukaryotic organisms.
Structure and function of the invasion apparatus of microsporidia
Dohnálek, Vít ; Doležal, Pavel (advisor) ; Vávra, Jiří (referee)
Microsporidia are obligate intracellular parasites that cause significant economic damage and can infect humans. They had evolved highly specialized invasion apparatus that is unique to them. During the invasion the sporoplasm is released from the spore wall and it is transferred to the host cytoplasm through the polar tube. Current research on the invasion apparatus has been focused mainly on the polar tube that is its most prominent structure. Polaroplast and posterior vacuole remain out of the main interest, although they are necessary for the activation and execution of the invasion. If the right combination of environmental factors occurs, the organization of the polaroplast begins to change, the posterior vacuole starts to swell and the polar tube is discharged. Sporoplasm is eventually pushed through the tube into the host cell by growing posterior vacuole. The mechanism has not been explained yet however plenty of theories are trying to explain the germination. This work summarizes current theories and knowledge of structure and function of particular parts of the invasion apparatus. Key words: Microsporidia, invasion, polar tube, intracellular parasitism, germination

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