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Analysis of tourism in the archaeological site of Machu Picchu
Břežná, Tereza ; Vilímek, Vít (advisor) ; Klimeš, Jan (referee)
This bachelor thesis is an overview of the analysis of tourism in the archaeological monument of Machu Picchu with a focus on overturism. Tourism is an ever-evolving sector that brings both positive and negative aspects to places, such as overturism, which is also on Machu Picchu. The work also presents a brief history of the object and life of the Incas and the basic physical and geographical setting of the place, including natural hazards. The main goal of this work is the analysis of tourism at Machu Picchu and its development in the period 1980-2019. The research is also focused on the analysis of attendance of tourists during the year in correlation with weather and air transport. Part of the analysis focuses on research of the number of tourists in acreage and the population of Machupicchu village. The aims of the work also deal with the regulatory mechanisms and comparison of Machu Picchu with other UNESCO monuments. The bachelor thesis uses methods of calculating the number of tourists divided by the area in m2 and the number of tourists divided by the population of Machupicchu. Machu Picchu is a heritage site that has been burdened by overtourism in a long period of time. Its attendance is rising sharply every year, in 2019 it was visited by over 1.5 million tourists from around the world....
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Development and validation of method for determination of furosemide and its degradation product in pharmaceutical formulations
Břežná, Tereza ; Matysová, Ludmila (advisor) ; Sklenářová, Hana (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Tereza Břežná Advisor: PharmDr. Ludmila Matysová, Ph.D. Thesis name: Development and validation of method for determination of furosemide and its degradation product in pharmaceutical formulations This thesis is focused on development and validation of a method for determination of furosemide and its degradation product in pharmaceutical formulations. The development was based on the HPLC method, which uses the mixture of 0.1 % formic acid and methanol in the 30:70 ratio as the mobile phase. The method development has been namely based on the mobile phase composition and analysis temperature conditions optimizing. In the final version was used the HPLC method with UV-VIS detection at 270 nm. The analysis was performed at the laboratory temperature using isocratic elution and C18 15 x 4.6 mm column with 5 μm particle size. The mixture of pH 5.75 formic acid and acetonitrile in the 65:35 ratio and at 1.5 ml/min flow rate has been selected as the mobile phase. The validation has proven that this method provides precise and acurate results and therefore it is suitable for furosemide determination in the solutions of medicinal products.
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Development and validation of method for determination of furosemide and its degradation product in pharmaceutical formulations
Břežná, Tereza ; Matysová, Ludmila (advisor) ; Sklenářová, Hana (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Tereza Břežná Advisor: PharmDr. Ludmila Matysová, Ph.D. Thesis name: Development and validation of method for determination of furosemide and its degradation product in pharmaceutical formulations This thesis is focused on development and validation of a method for determination of furosemide and its degradation product in pharmaceutical formulations. The development was based on the HPLC method, which uses the mixture of 0.1 % formic acid and methanol in the 30:70 ratio as the mobile phase. The method development has been namely based on the mobile phase composition and analysis temperature conditions optimizing. In the final version was used the HPLC method with UV-VIS detection at 270 nm. The analysis was performed at the laboratory temperature using isocratic elution and C18 15 x 4.6 mm column with 5 μm particle size. The mixture of pH 5.75 formic acid and acetonitrile in the 65:35 ratio and at 1.5 ml/min flow rate has been selected as the mobile phase. The validation has proven that this method provides precise and acurate results and therefore it is suitable for furosemide determination in the solutions of medicinal products.
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