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Transition from alternative kindergarten to elementary school
NÍDLOVÁ, Pavlína
bachelor's thesis is divided into two parts, a theoretical and a practical one. The theoretical part is focused on alternative education available in the Czech Republic. It explains the basic principles of alternative pedagogical concepts and also clarifies the concept of contemporary traditional education in the Czech Republic. The practical part presents the results of a research investigation conducted in the form of interviews. Their aim was to describe the experiences of children with the transition from an alternative kindergarten to a traditional primary school, and to get an idea of the problems, or on the contrary, the advantages of adapting to a different type of school.
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Implementation of Fracture Liaison Service in the Czech Republic
VRABLÍKOVÁ, Květa
The main goal of the rigorous work was to verify the feasibility of implementing a coordinated care program for patients after osteoporotic fractures (so-called low traumatic fractures) of the so-called Fracture Liaison Services (FLS) system in the Czech Republic from the point of view of nurses' access. Sub-objectives of the quantitative research mapped the current status of adherence to procedures in the care of patients after low-traumatic fracture from the perspective of nurses, attitudes of nurses towards implementing FLS, and access to continuing education for the position of FLS coordinator. The sub-objectives of the qualitative research compared the development of care for osteoporotic patients in the Czech Republic between 2010 and 2019. They compared the situation in Lithuania, Romania, and Slovakia between 2010 and 2019.
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Regenerace smyslových orgánů u raků
KOR, Golara
The antennae are a crucial sensory organ in crayfish that plays vital roles in their survival, communication, and orientation within their environment. They are equipped with sensory hairs that enable crayfish to detect various stimuli. Crayfish possess the unique ability to regenerate their antennae. Hemocytes are specialized cells within crayfish and play a crucial role in their immune system and contribute to the process of regeneration. We applied cryo-scanning and Transmission electron microscopies to study sensory organ morphology and regeneration in crayfish. In Chapter 2, cryo-scanning electron microscopy was used to identify potential differences in antennal morphology in six different crayfish species including marbled crayfish Procambarus virginalis, Mexican dwarf crayfish Cambarellus patzcuarensis, red swamp crayfish Procambarus clarkii, signal crayfish Pacifastacus leniusculus, common yabby Cherax destructor, and spiny-cheek crayfish Faxonius limosus. We observed significant differences among the six crayfish species in the ratios of antenna length, segment length and width to carapace length, and the number of segments. Examination of ultrastructural features unveiled differences in the distribution patterns of sensory hairs along the antenna and the morphology of the antennal surface. The varying morphology of antennae among studied species possibly indicates an adaptation to the specific conditions of their respective habitats. Furthermore, the outcomes demonstrated that a combination of variations in both antennal morphological characteristics and biometric measurements could effectively help us to distinguish the different studied crayfish species. In Chapter 3, the ultrastructural behaviour of hemocytes during coagulation and phagocytosis in the initial phases of injury in marbled crayfish was explored using transmission electron microscopy. During the coagulation process, hemocytes experienced marked transformations in morphology. The cytoplasmic granules exhibited a change from electron-dense to electron-lucent forms with the progress of coagulation. The transformed granules containing amorphous, electron-lucent material were observed to combine and release their contents into the extracellular space as part of the coagulation process. Additionally, it was observed that the nucleus contents also play a role in the coagulation process. Furthermore, the amputation of the leg led to substantial muscle degeneration, and phagocytic hemocytes start to take up the necrotic tissues. Besides, we observed the digested remains from phagocytized necrotic tissues merged into granules and other cellular components, thereby enhancing the granularity of the hemocytes and altering their cellular morphology. However, it is essential to note that hemocyte degranulation during coagulation could potentially reduce their granularity. Since morphological features are critical for classifying hemocytes, these morphological changes during coagulation and phagocytosis must be considered significant factors. The degenerated material that stored inside phagocytic hemocytes may have application in immunological and regeneration processes. In Chapter 4, we employed transmission electron microscopy to explore the ultrastructural aspects of potential immune cell involvement in nerve regeneration within crayfish antennae post-amputation. The findings indicated that, during nerve regeneration, all three forms of hemocytes were present. However, the granules within semi-granulocytes and granulocytes mainly contributed to the generation of new organelles such as mitochondria, the Golgi apparatus, and nerve fibers in the regenerated nerves of crayfish antennae. We revealed the transformation of granules of hemocytes into diverse organelles during the process of nerve regeneration at the ultrastructural level. In conclusion, these granules function as compact repositories of adaptable materials carried by immune cells, capable of transformatio
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Investigating associations of saproxylic beetles with forest structure through the analysis of species composition and feeding guilds
KOZEL, Petr
The thesis contains studies focusing on various forest biotopes of Central Europe and saproxylic beetles as a model group for forest ecology research. In the review, current knowledge about the threats for forest biota, mainly saproxylic beetles are discussed in the context of species composition and trait-based approach. Various stages of succession in the patches of disturbed forests, their connectivity, the introduction non-native tree species, and the assessment of feeding guilds using stable isotopes are studied in the relation to saproxylic beetles in separate chapters.
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Anthropogenic impact on early-life stages of rheophilic fish
BARTOŇ, Daniel
This study focuses on the problems faced by the early-life stages of fish living in a river influenced by reservoirs and suggests possible solutions to enhance recruitment. The model fish for the study is the asp (Leuciscus aspius), known for its significant and protected population in the Želivka Reservoir, from where it migrates for reproduction to the tributary of Želivka River. The asp migrates upstream in spring to spawn in shallow, fast-flowing waters. At a research site, the adult population of asp is monitored using passive telemetry. Fish are caught, tagged, and released back into the reservoir. The asp population faces threats due to hydropeaking and river fragmentation. Hydropeaking causes egg detachment from the pebbles and their sedimentation in the reservoir. The study suggests that water should not exceed a velocity of 0.7 m×s-1 at the spawning ground. Periodically increasing discharge also shifts spawning fish outside their preferred spawning site, causing disruption of spawning and potentially lowering recruitment. A flowdeflector was built to protect newly spawned eggs from hydropeaking. However, there are additional threats to the asp recruitment process such as egg consumption by non-indigenous fish like common bream (Abramis brama). Water flow conditions are crucial for the conservation of rheophilic fish, as these fish, aside from habitat loss and modification, also face newly interacting generalist fish species moving from lentic to lotic sections of the system. Fish communities can be affected by reservoir construction far upstream, and these anthropogenic habitat alterations have severe negative impacts on threatened rheophilic fish recruitment.
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The study of the phylogeny and taxonomy of selected genera of Erebidae (Lepidoptera; Noctuoidea) as a tool for understanding of origin of the Afrotropical moth fauna
IGNATEV, Nikolai
In this thesis, I conducted the taxonomical and phylogenetical research of selected Afrotropical moth genera, especially an Old-World moth genus Amerila, Walker 1855. All the studied genera belong to the family Erebidae (Lepidoptera; Noctuoidea) and are well-presented in the state European entomological collections. In our research, we used mitochondrial, or mitochondrial and nuclear markers and different molecular analyses to resolve relationships within the studied genera (exclude Meganaclia, Aurivillius 1892). Comparative morphological analysis was used in all the studies. We aimed to update the classification of studied Lepidoptera genera, and to highlight the gaps in taxonomical study of Afrotropical moth fauna in general.
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Application of genetic algorithms for optimisation of agricultural machinery operation in precision farming systems
BUMBÁLEK, Roman
Metaheuristic methods, inspired by physical, biological, chemical, semantic and social phenomena, are used to optimise problems with a large number of possible solutions in a wide range of fields, including agriculture, with a significant part of evolutionary computation, of which genetic algorithms are mainly used for precision agriculture. This paper deals with their implementation in the context of the problem of planning the routes of agricultural machines with the aim of reducing the length of the trajectory of movement both on the headlands and in the inner part of the plot. The developed algorithm consists of several parts, where first the coordinates of the partial routes are calculated and then genetic algorithms are applied to optimise the rotation of the im-plements on the headlands through a suitable combination of their passes with respect to the input parameters, including the turning radius and the tool sweep. The quality of the results obtained is influenced by many factors, such as the size of the population, the number of generations or the type of selection chosen, with better solutions being generated as the number of chromosomes in the population and generations increases, allowing a reduction of more than 37 % in the distance travelled when turning on he-adlands, which can directly translate into savings in working time, fuel and reduced soil compaction.
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Computer modelling of the movement of agricultural machinery during field
FILIP, Martin
This dissertation deals with the problem of optimizing the number of hauling trailers and loaders in the handling, collection and transport of bales of fodder or straw from the land to the storage site in order to achieve the lowest possible consumption of human labour. The automated decision-making process allows the optimum number of removal trailers and loaders to be selected s that there is no unnecessary downtime for the handling equipment while waiting for the transport equipment or, conversely, no lost time for the removal trailers due to insufficient loader capacity. The basic function of the optimization program is to process a Google Maps image of a real plot of land, detect its boundaries and, on the basis of estimates of forage or straw yields, estimate the approximate distribution of predefined bale sizes and determine the centroids, i.e. the locations where the hauler should be positioned when loading.The centroid is also the point from which the shortest travel distances for the loader are obtained. The centroids are assigned successively to the packages with the lowest distance until the group is full. This is the condition where the number of bales in the group corresponds to the transport capacity of the conveyor. Another function of the optimization algorithm is to schedule the travel routes in the centroid and based on the input value of the average speed of the loader, the time required for the travel of this machine is calculated. To the total working time of the loader is added the time required for driving from the storage area of the machine to the plot, as well as the time required for driving from the point of entry to the working area to the centroid, and the driving time from the processed centroid to the other centroids, including the driving time from the harvested areas back to the place where the loader will be parked. In addition, the value of the time required to lift all bales in the centroids and place them on the conveyor and the downtime of the manipulator are added to the loader's travel time to obtain the loader's total time. The working time of the removal trains is calculated from the distance of the route (multiplied by the average speed of these machines) from the storage location of the machines to the centroid, the loading time and the time required to travel to the storage location of the bales, including the time required to remove the bales from the removal trains. The time of the loaders and the removal trains is evaluated simultaneously, in synchronisation, and it is calculated whether there are any lost times, which are added successively to the working times. The optimization program sequentially calculates the different variants for (n) loaders, (n) transfer sets, evaluates which variant consumes the least time and is therefore optimal in terms of the lowest time consumption.
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Metabolic setup of Drosophila macrophages during the immune response
KREJČOVÁ, Gabriela
Adjustment of cellular metabolism is a key function that allows macrophages to fulfill their roles in the body. While the pro-inflammatory polarization of macrophages has been extensively studied in mammalian models, it has not yet been satisfactorily investigated in insects. The study presented in this thesis therefore attempts to elucidate the metabolic setup of macrophages during the immune response in Drosophila melanogaster.
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