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Computer modelling of the movement of agricultural machinery during field
FILIP, Martin
This dissertation deals with the problem of optimizing the number of hauling trailers and loaders in the handling, collection and transport of bales of fodder or straw from the land to the storage site in order to achieve the lowest possible consumption of human labour. The automated decision-making process allows the optimum number of removal trailers and loaders to be selected s that there is no unnecessary downtime for the handling equipment while waiting for the transport equipment or, conversely, no lost time for the removal trailers due to insufficient loader capacity. The basic function of the optimization program is to process a Google Maps image of a real plot of land, detect its boundaries and, on the basis of estimates of forage or straw yields, estimate the approximate distribution of predefined bale sizes and determine the centroids, i.e. the locations where the hauler should be positioned when loading.The centroid is also the point from which the shortest travel distances for the loader are obtained. The centroids are assigned successively to the packages with the lowest distance until the group is full. This is the condition where the number of bales in the group corresponds to the transport capacity of the conveyor. Another function of the optimization algorithm is to schedule the travel routes in the centroid and based on the input value of the average speed of the loader, the time required for the travel of this machine is calculated. To the total working time of the loader is added the time required for driving from the storage area of the machine to the plot, as well as the time required for driving from the point of entry to the working area to the centroid, and the driving time from the processed centroid to the other centroids, including the driving time from the harvested areas back to the place where the loader will be parked. In addition, the value of the time required to lift all bales in the centroids and place them on the conveyor and the downtime of the manipulator are added to the loader's travel time to obtain the loader's total time. The working time of the removal trains is calculated from the distance of the route (multiplied by the average speed of these machines) from the storage location of the machines to the centroid, the loading time and the time required to travel to the storage location of the bales, including the time required to remove the bales from the removal trains. The time of the loaders and the removal trains is evaluated simultaneously, in synchronisation, and it is calculated whether there are any lost times, which are added successively to the working times. The optimization program sequentially calculates the different variants for (n) loaders, (n) transfer sets, evaluates which variant consumes the least time and is therefore optimal in terms of the lowest time consumption.
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Metabolic setup of Drosophila macrophages during the immune response
KREJČOVÁ, Gabriela
Adjustment of cellular metabolism is a key function that allows macrophages to fulfill their roles in the body. While the pro-inflammatory polarization of macrophages has been extensively studied in mammalian models, it has not yet been satisfactorily investigated in insects. The study presented in this thesis therefore attempts to elucidate the metabolic setup of macrophages during the immune response in Drosophila melanogaster.
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The role of macrophages in the regulation of systemic metabolism in Drosophila
KREJČOVÁ, Gabriela
Macrophages are immensely versatile cells in the mammalian body, fulfilling roles ranging from protection against pathogenic intruders and engulfing apoptotic cells to morphogenesis and maintenance of tissue homeostasis. This impressive functional versatility may be achieved due to plasticity of macrophage cellular metabolism called metabolic polarization. The adoption of different polarization phenotypes by macrophages determines their function and is essential for the health of the organism. Nonetheless, if the cells lose their metabolic plasticity or polarize inadequately to a particular situation, it can lead to the development of chronic pathological states such as metabolic syndrome. Metabolic polarization of immune cells is thus a key factor in determining whether macrophage function within the organism will be adaptive or pathological. Despite Drosophila melanogaster represents a major model organism for immunological studies, the metabolic setup of activated immune cells has not been addressed up to now. The results of this thesis document that Drosophila immune cells undergo metabolic polarization toward aerobic glycolysis when challenged by extracellular bacteria. Mammals alike, this cellular metabolic switch is regulated by the transcription factor HIF1, thus documenting the conservation of this process between insects and vertebrates. Furthermore, we show that the adoption of aerobic glycolysis is directly linked to the production of the signaling factor IMPL2, which induces the mobilization of lipid stores from the fat body via the silencing of insulin signaling. By this mechanism, immune cells secure sufficient nutrients for successful elimination of the pathogen. Moreover, the mammalian ImpL2 homolog IGFBP7 appears to act analogously in the mammalian liver not only during severe infectious states but also in the liver of obese individuals. While such macrophage activity in regulating systemic metabolism is beneficial to the host during bacterial infection, it becomes maladaptive when chronically activated. Further evidence for a metabolism-regulatory role of immune cells has been found during insect metamorphosis and early post-metamorphic development. This thesis documents that during this period, macrophages infiltrate and engulf the histolyzing larval fat body and convert nutrients into storage peptides and lipoproteins. Subsequently, these nutrients are exploited by the maturing adult structures.
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Functional Ultrastructure of Hymenopteran Stingers: Devastating Spear or Delicate Syringe
ČERNÝ, Jan
In this study, we tested the hypothesis that a micro-serrated edge on the honey bee Apis mellifera stinger tip serves as a tool for more intensive crushing of cell membranes in the victim's tissues. This could have mechanical consequences as well as initiate metabolic pathways linked to cell membrane breakdown (e.g., production of biogenic amines). Accordingly, we found that hymenopteran species that use their stingers as an offensive or defensive weapon to do as much damage to the victim's body as possible had this cuticular microstructure. In parasitic hymenopterans, on the other hand, this structure was missing, as stingers are solely used to delicately transport venom to the victim's body in order to do little mechanical harm. We also demonstrated that the stinger lancets of the honey bee A. mellifera are living organs with sensilla innervated by sensory neurons and containing other essential tissues, rather than mere cuticular structures.
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Importance of environmental elements (water environment and greenery) in urbanized areas of the Czech Republic
HAVRÁNKOVÁ, Lucie
The aim of the dissertation is to provide an analytical comparison of the effectiveness of using of the environmental potential of watercourses and greenery in urbanized areas/cities of the Czech Republic (ČR). The work is divided into a part of physical-geographical and sociological assessment and research. The physical-geographical analysis will provide the results of watercourse use in the city using the Urban River Sustainability Index (URSI) and watercourse availability analysis. In the area of eva-luation of cities in terms of greenery, the results are obtained through the calculation of the Indicator of the General Quality of Greenery in Cities (IOKZM) based on the Coefficient of Ecological Stability (KES) and the evaluation of the share of greenery in cities. Sociological research provides the user's opinion on the positive environmental contribution of the watercourse in specific urbanized areas, based on the results of quantitative and qualitative research. This section is statistically evaluated using logistic regression (LR) and multivariate correspondence analysis (CA). The overall results of the work create an overal image of the importance of environmental elements (water environment and greenery) in urbanized areas of the Czech Republic. They draw attention to the current state of Czech cities and suggest possible improving solutions not only for their changing climatic situation.
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The influence of the Second Vatican Council on the Church's attitude to the world. Illustration on the theology of church history in the Czech lands during communism
OTÝPKOVÁ, Petra
The dissertation deals with impact of Second Vatican Council in attitude of the Catholic Church to World and it is illustrated in life of the Church in Czech countries in the course of communistic period, because of marginal differances between Church and World. Read the sings of times and aggiornamento are key concepts of Second Vatican Council. Main source are documents of Council and works dedicated to Second Vatican Council, what give us the most comprehensive information. Situation of the Church in the Czech countries from the point of view of systém of government, what effort to eliminate the Church, and response of the Church to it, what impacted reception of Second Vatican Council, and it is illustrated in thematic works and contemporary witness of communist regime. Generaly impact of Second Vatican Council find to reflection directed to situation of the Church and society and the ways, what Church choices for spreading of Gospel. In the Czech countries the Second Vatican Council encourage catholics to be active and take responsibility for civic society.
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Development of theological discourse at Dorothea Sölle
BALI, Kateřina
The presented work deals with the theological reflection of the ideas of Protestant, feminist theologian Dorothee Sölle, related to her work Mysticism and Resistance. The aim of this work is to reflect on the concepts of mysticism and resistance, while viewing mysticism and resistance in direct relation to Sölle's position within feminist liberation theology, i.e. attempting to place ideas about mysticism and resistance in the framework of her early theological thinking. With regard to Sölle's position within feminist theology, the work also deals with the question of how mysticism and resistance take on the future form of feminist liberation theology. The focus of this work, through the hermeneutic method, is mainly the reflection of the relationship between action and contemplation, standing at the core of mysticism and resistance. Against the background of Zimmerling's criticism of mysticism and resistance, primarily from the politicization of mysticism, the work tries to reflect on the relationship between mysticism and resistance, which is a relationship of radical correlation. Zimmerling's criticism seems justified in view of the focus on relationships within creation and the related transformation of man, instead of the relationship with God. In a certain respect, mysticism as resistance takes on the form of political mysticism, more precisely the mysticism of liberation. The reflection of mysticism as resistance raises questions about the "need" of the "contemplative dimension", which as a result can appear secondary. The work tries to find answers in Sölle's text, and that answer can be the reflection of aimless prayer, which is the "heart" of mysticism as resistance. Although Sölle emphasizes one aspect of the mystical relationship in particular - the transformation of man and the world, her goal is the unity of action and contemplation in in the form of the unity of mysticism and resistance. In her perspective, the relationship with God is not an isolated experience of an individual, but a reflection and at the same time a pattern of relationships within creation. Just as it does not separate action from contemplation, it does not separate the relationship between God and man and man and creation. As a result, Sölle's desire to find unity can be found in the background of this approach. It is precisely in the idea of mysticism as resistance, in the mysticism of open eyes, that the dialogical relationship of action and contemplation emerges, which is a mutual relationship. Action and contemplation are two sides of the same coin, i.e. one cannot exist without the other, and their harmony is a prerequisite (and consequence) of mysticism and resistance.
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Diagnostika parazitárních onemocnění a úspěšnost vybraných terapeutických postupů u lichokopytníků a sudokopytníků
HAVRDOVÁ, Nikola
This dissertation dealt with the diagnosis and therapeutic procedures for the treatment of parasitic diseases in odd-toed and even-toed ungulates with selected anthelmintics. Between 2020 - 2023, 5.359 artiodactyls and 819 solipeds, namely cattle, sheep, goats, aurochs, mouflon, deer, bison, bison bonasus, horses, donkeys and ponies, were microscopically examined for the presence of nematodes, trematodes or cestodes in faecal samples in the Czech Republic. Molecularly, 76 cattle samples, 26 bison bonasus samples and 52 bison samples were examined for the presence of trematodes and C. daubneyi was detected. Parasitic infections were monitored in naturally infected individuals, and during the study no clinical signs suggestive of parasitemia were observed in any of the monitored individuals. The most parasitic infections were evaluated for H. contortus, O. ostertagi, M. benedeni, C. daubneyi and A. perfoliata. As part of the dissertation, a total of ten anthelmintics available in the Czech Republic were chosen, focusing on individual categories of parasitic infections in various animal species. Based on laboratory analyzes of LDA tests, the species of nematode was determined according to the developmental stage of the larvae, which helped to choose the appropriate anthelmintic during treatment. In conclusion, it can be stated that almost all anthelmintics have been shown to be effective in therapeutically treated farm animals.
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A novel structurally characterized haloacid dehalogenase superfamily phosphatase from Thermococcus thioreducens with diverse substrate specificity
HAVLÍČKOVÁ, Petra
This RNDr. thesis is focused on complex studies of a haloacid dehalogenase superfamily phosphatase member with an unknown substrate specificity, isolated from a hyperthermofilic archeon Thermococcus thioreducens. The HAD phosphatase, internally named as Tt82, was isolated, expressed and purified using standard techniques and the crystal structure was solved by X-ray crystallography method. The refined crystal structure displayed characteristic HAD superfamily elements, such as the HAD canonical fold with the HAD signature motifs in the active site. Further, possible substrates for the enzyme were proposed and examined by computational docking in the active site of the HAD phosphatase. The results of the docking studies served as a basis for an experimental measurement of Tt82 enzymatic activity against selected substrates at two different temperatures. HAD phosphatase Tt82 is a promising target for the biotechnological field, mainly for its wide substrate specificity and higher temperature optima.
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Functional characterization of the insulin signaling pathway in the hard tick Ixodes ricinus
KOZELKOVÁ, Tereza
In this thesis, the molecular and functional characterization of the insulin receptor signaling pathway (ISP) in hard tick Ixodes ricinus was characterized. As obligatory blood-feeding ectoparasites, ticks play a crucial role in the transmission of various pathogens, including bacteria, viruses, and protozoa, which has a significant impact on human and animal health. The parasite is strictly bonded with its host through a unidirectional transmission of nutrition for its survival, development, and reproduction. The ISP, a highly conserved system, regulates numerous physiological and anabolic processes to nutritional availability. This study aims to investigate the functionality of key components of the ISP pathway identified in the midgut transcriptome, namely the insulin receptor (IrInR), the protein kinase B called AKT (IrAKT), and the target of rapamycin (IrTOR). To achieve this goal, the expression profiles of these components in tick tissues during feeding and after detachment were investigated using qRT-PCR. Additionally, RNAi silencing of individual components was performed, and the phenotype of the ticks was observed. To further clarify the effects of ISP, immunization of rabbits with recombinant IrInR protein and tick infestation were investigated.
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