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National Repository of Grey Literature

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The used of magnetic microparticles for isolation and prove of probiotic bacterial DNA in meat Vašíček, Roman ; Rittich, Bohuslav (referee) ; Španová, Alena (advisor) This thesis deals with the isolation of probiotic DNA from meat products and its assesment by PCR methods. In this thesis is developed homogenization of samples of sausages with kopist, preparation of sausage cells lysates and isolation of DNA by using of magnetic microparticles. The DNA was isolated from sausage lysates by using magnetic microparticles. Isolated DNA was further amplified in genus and spesies-specific PCR methods. In tested products was proven presence of DNA of domain Bacteria, type Lactobacillus and Bifidobacterium. In one product was proven presence of species Lactobacillus acidophilus and Bifidobacterium animalis. Detailed record

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