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Identification of phenolic compounds in plant extracts using UHPLC-MS/MS
Kmeťová, Ivana ; Nováková, Lucie (advisor) ; Chocholouš, Petr (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate: Ivana Kmeťová Supervisor: Doc. PharmDr. Lucie Nováková, Ph.D. Title of Diploma thesis: Identification of phenolic compounds in plant extracts using UHPLC-MS/MS The aim of this Diploma thesis was identification of phenolic compounds in dry lyophilized water extract of leaves of wild strawberry (Fragaria vesca L.) using ultra-high performance liquid chromatography coupled with electrospray tandem mass spectrometry (UHPLC-ESI-MS/MS), advantageous in identification of complex plant samples. Phenolic compounds represent a group of very complex naturally occurring molecules in plants, possessing a wide variety of biological and physiological functions and acting as chemotaxonomic marker substances. This work was focused on flavonoids and related substances. For separation, UHPLC was used instead of more common HPLC due to its much higher separation efficiency that is crucial for successful separation of complex samples. Moreover, UHPLC enabled reduction of analysis time. Determination of structures of phenolics was based on evaluation and comparison of MS and MS/MS spectra in both negative and positive ESI ion modes. Full scan spectra, product ion spectra, neutral loss scans and reconstructed...
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