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Comparison of columns for separation of pharmaceutical substances by method sequential injection chromatografie
Vacková, Jana ; Chocholouš, Petr (advisor) ; Matysová, Ludmila (referee)
Charles University in the city of Prag Pharmaceutical fakulty in the city of Hradec Králové Department of the Analytical Chemistry Consultant: PharmDr. Petr Chocholouš, PhD. Candidate: Jana Vacková Name of the thesis: Comparsion of columns for separation of pharmaceutically significant substances by method of Sequetial Injection Chromatography This diploma thesis deals with the development of the separation method in the system of the Sequential Injection Chromatography (SIC). The used SIC system was based on the system SIChrom™ (FIAlab® , Bellewue, WA, USA) with the eight port stainless steel multi-position valve (VICI® Valco Instruments, TX, USA) and with the pump S17 (Sapphire™ Engeneering, MA, USA). The samples were prepared by dissolving of 25 mg of various phenolic acids (protocatechuic acid, vanillic acid, p-coumaric acid, ferulic acid, o-coumaric acid, sinapinic acid and syringic acid) in 25 ml of methanol. Three fused-core particle columns were used in this thesis : the column Ascentis Express C-18 (30 mm × 4.6 mm, 2.7 µm, Sigma - Aldrich, Supelco Analytical, Bellefonte, PA, USA), the column Ascentis Express RP-Amide (30 mm × 4.6 mm, 2.7 µm, Sigma - Aldrich, Supelco Analytical, Bellefonte, PA, USA) and the column Ascentis Express Phenyl-Hexyl (30 mm × 4.6 mm, 2.7 µm, Sigma - Aldrich,...
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