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Application of electromigration methods in the analysis of Francisella tularensis proteins
Žáková, Jitka ; Konečná, Klára (advisor) ; Jílek, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Degree program: Medical bioanalysis Field of study: Medical Laboratory Technician Author: Jitka Žáková Title: Application of electromigration methods in the analysis of Francisella tularensis proteins Bachelor thesis Theoretical part describes pathogenic bacterium Francisella tularensis (taxonomy, morphology, cultivation, mode of disease, sources of infection, epidemiology) and basic characterisation of electromigration methods (electrophoresis, izoelectric focusing, izotachophoresis, capillary electrophoresis). Experimental part contains methods and results of selected electromigration experiments (1DE SDS-PAGE, 2 DE SDS-PAGE). We chose the methods that prove to be usefull in generating data suitable for current publication in international journals, thus ilustrating the significance in analysis of F. tularensis proteome.
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