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Immunofluorescent labeling of tissues and free cells via QD-protein conjugates
Lišková, Marcela ; Voráčová, Ivona ; Hezinová, Věra ; Klepárník, Karel ; Foret, František
Preparation of quantum dots and using their QD-protein conjugates in immunofluorescent labeling of cells and tissues. For conjugation was used method viaEDC/sulfo-NHS zero-length cross-linkers. We linked QD with annexin V, anti-CD3 and anti-PCNA. We labeled T-lymphocytes, apoptotic lymphocyte, mouse duodenum tissue and tumor tissue.

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