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National Repository of Grey Literature

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Influence of glutamate-induced obesity on detoxification enzymes contains glutathione Duchačová, Kateřina ; Boušová, Iva (advisor) ; Szotáková, Barbora (referee) Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Kateřina Duchačová Supervisor: doc. PharmDr. Iva Boušová, Ph.D. Title of rigorous thesis: Influence of glutamate-induced obesity on detoxification enzymes contains glutathione Obesity and related comorbidities occur more frequently in the population and belong to the most studied contemporary health topics. Aging of the organism is much discussed topic as well. Various animal models are used to observe changes in the organism at the organ, tissue, cellular and molecular levels. Comparison of specific activities and protein expressions of glutathione peroxidase (GPx) and glutathione reductase (GR), and specific activity of glutathione-S-transferase (GST) in the liver of obese mice and healthy controls according to the pathological state, aging and sex was the aim of this study. Mice with monosodium glutamate (MSG)-induced obesity, osmolal controls (NaCl) and mice without treatment of both sexes and at 8 and 20 weeks of age were used. Specific enzyme activities were determined spectrophotometrically. Protein expressions were determined using polyacrylamide gel electrophoresis followed by immunoblotting. The biggest obesity-related changes in enzyme activity were observed in GST, which activity... Detailed record

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