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Abiotic Elicitation of Suspension Culture of Trifolium Pratense L.
Vaňková, Jitka ; Kašparová, Marie (advisor) ; Siatka, Tomáš (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Mgr. Jitka Vaňková Supervisor: PharmDr. Marie Kašparová, Ph.D. Title of Rigorous Thesis: Abiotic elicitation of suspension culture of Trifolium pratense L. We investigated the effect of a 24-hour application of aluminum chloride elicitor alone (1 mol, 10 mol, 100 mol) and in combination with calcium chloride (0,1 mmol, 1 mmol, 10 mmol) and verapamil (1 mol, 10 mol, 100 mol) on the production of flavonoids and isoflavonoids in 4-year-old and newly derived suspension cultures of Trifolium pratense L. Cultures were cultivated on a medium according to Gamborg added 2,4-dichlorophenoxy acetic acid (2 mg.l-1 ) with 6-benzylaminopurine (2 mg.l-1 ) at 25 ř C and a light period of 16 hours light / 8 hours dark. The maximal production of flavonoids (0.262% and 0.220%) resulted in two observed groups of cultures add the weakest concentration of aluminum chloride (1 µmol) and calcium ions (0.01 mmol). Subsequent addition of elicitation effect of verapamil as a calcium channel blocker has been demonstrated by reducing the production of metabolites observed in both cultures only after the strongest concentration of 100 µmol. The newly derived suspension cultures of Trifolium pratense L. was also...
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