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Phospholipase D Beta in hormonal signaling of Arabidopsis Thaliana
Krutinová, Hana ; Ryšlavá, Helena (advisor) ; Hýsková, Veronika (referee)
Plants, as well as all other living organisms, have to react to environmental changes and are forced to put up with the danger that comes from their environment. As a response to this danger, a sophisticated defense system, which moderates responses to stress cues, comes from the outside as well as created inside the plant itself, has evolved in plants. The signaling in this system is mediated by a number of phytohormones, which include salicylic acid, jasmonic acid, abscisic acid, ethylen and auxins. The phytohormone signaling results in a transcription of specific genes. One of the essential components of this signaling network is the phospholipid signaling system, where enzymes called phospholipases play a major role. These enzymes catalyze the hydrolysis of phospholipids, which are part of cellular membranes and products of the hydrolysis can act as signal transferring molecules. In this thesis we studied the potential engagement of phospholipase Dβ isoform into signaling pathways, using an Arabidopsis thaliana mutant plant with mutations in both genes coding phospholipase Dβ. Firstly, we verified that wild type phospholipase Dβ mRNA is not being transcribed in the mutant plants. Subsequently, we compared mutant and wild type plants' reactions to mechanical wounding, pathogen infection and...
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