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Rapid detection microogranism in pharmaceutical materials.
Novosád, Aleš ; Jílek, Petr (advisor) ; Voxová, Barbora (referee)
1 Summary Many microbiology laboratory, no matter what already in pharmaceutical firms or to hygienic stations ground and firms conversant food production used to analysis big quantity microbiology samples conventional techniques based on cultivation appropriate culture. These techniques take hours to days to yield a result, are tedious and are not suitable for non- culturable microorganisms. Further, culture-based techniques do not provide real-time information on the physiological status of the organism in situ which is important in the industrial manufacture of many microbial products (1) and often at high of the number of micro - organisms, when isn't possibility number micro - organisms determine, all time cultivation about next dilution sample elongate and about time growth this micro - organism. Flow cytometry makes it possible to obtaining real microbial determination single micro - organisms, without dependencies on type microbial culture. Nevertheless, flow cytometry has not been extensively used as a tool for routine microbial analysis. Reason is mainly high cost and complexity of instrumentation, the need for trained flow cytometrists and the lack of assay kits with appropriate biological reagents for specific applications. Much modern instruments are now relatively simply serviceable, thanks...
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