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Laboratory diagnosis of Candida infections
 Vejsová, Marcela ; Buchta, Vladimír (advisor) ; Mencl, Karel (referee) ; Hamal, Petr (referee)
Pathogenic Candida species represent the most common fungal etiology of human opportunistic infections, particularly among hospitalized patients. After decades of increasing morbidity and mortality of candidiasis, the proportion of Candida infections had become to stop and the shift of spectrum of Candida species has started to be evident. Apart from a predominating position of C. albicans, there is a growing number of non-albicans Candida species (e.g. C. glabrata, C. tropicalis) as causative agents of yeast infections. Mycologists are faced up new challenges of rapid detection and precise identification of fungal isolates including antifungal susceptibility evaluation. Goal of the study was to confirm usefulness of rapid tests in laboratory diagnostics of C. albicans and elaborate an alternative method of classical germ tubes test in serum. The results of experiments showed the use of chromogenic media useful and reliable as well as the test of induction of germ tubes. The comparative tests showed that serum can be replaced with chemically defined NYP medium without loss of high of specificity and sensitivity of the test. Main part of experiments was focused on optimalization of methodology of in vitro antifungal susceptibility testing. Etest and disk diffusion test are very reliable tests for...
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