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National Repository of Grey Literature

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Morphological characterization of monolithic columns Širc, Jakub ; Bosáková, Zuzana (advisor) ; Feltl, Ladislav (referee) ; Vacík, Jiří (referee) I ntroduction Iligh-perfbnnance liquid chromatography (lIPLC) is currently one of the ntost common anal1'tica| soparation 1cclrtlic1ttcs' Duc to its ability to separate a widc range oÍ'a structurally rlil.Íěrent lorr- and high ntolecular oompourtds o1'polar as rvcll as non-polar nature, tIPLC found application in analylical laboratories in thc field ofrnedicine. plrarrrracy. biology. industry and the environmelt. Although the oonverttional packed colunrns are used in most of ths chronratographic applications, stationary phases in the Íbrnr of.particles have their limitations and disadvňtages. Quality o| the packed colutltns is irrÍluerrced by a rrunrber ol. paranreters' |or exanrple relatiňly conrplicated process ol.lilling I l |' Variances in the particle size and inegularities in their shape have á n!'galive inrpact on thc chrotratoBraphic process. Conrmon problems of the packed oolunrns are an inhomogeucous distribution ol the particles ol'various sizes and their settling in the column u,ith consequent greation of dcad voIuntes' FIighly uniÍbm spherical partic|es prcpared by suspension polynrerization can partlY solve stlnte Iintitations. hor,vever. disadvantagcs arising Íionr the prin|iple of tlrc packcd coluurns still rcntain. Prornising altcmative to thc paoked colunrns are monoliths. made of a block... Detailed record

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