Home > Search Results: recid:280213
Search:
 
Search Tips :: Simple Search
Search collections:
Sort by: Display results: Output format:
National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 

(100)
Optimization of Electrophoretic Methods for Study of Glycation of Aspartate Aminotransferase by Methylglyoxal
 Bacílková, Eliška ; Szotáková, Barbora (referee) ; Boušová, Iva (advisor)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Eliška Bacílková Supervisor: PharmDr. Iva Boušová, Ph.D. Title of thesis: Optimization of electrophoretic methods for study of glycation of aspartate aminotransferase by methylglyoxal Main scope of this thesis was the optimization of several electrophoretic methods for monitoring of the course of glycation of aspartate aminotransferase (AST) by methylglyoxal as well as of the effect of selected antioxidants at this process. I observed formation of high-molecular protein aggregates using denaturing electrophoresis (SDS- PAGE) and Western blotting and changes in the molecular charge of AST during glycation using native PAGE. In both types of electrophoresis, I searched for suitable concentration of the separation gel and the amount of loaded protein. Further, I compared three routinely used staining methods (Silver Staining, Coomassie Blue R-250 and G-250). I found out that the high-molecular aggregates with molecular weight about 130 and 160 kDa, which corresponds to tri- and tetramers of AST, are formed with increasing concentration of methylglyoxal. Using immunochemical detection, I detected glycation products with molecular weight mainly about 70 and 130 kDa and with molecular...
Detailed record

Interested in being notified about new results for this query?
Subscribe to the RSS feed.
Digital Repository :: Search :: Submit :: Personalize :: Help
Powered by Invenio v1.1.2
Maintained by admin.invenio@techlib.cz
This site is also available in the following languages:
Česky  English