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Genista Tinctoria in vitro - the affecting of Secondary Metabolites Production
Danielová, Barbora ; Martin, Jan (referee) ; Tůmová, Lenka (advisor)
Genista tinctoria in vitro - the affecting of secondary metabolites production Elicitation is one of the methods that can be used for increasing secondary metabolites production in in vitro cultures. The present study investigates the effect of 6, 12, 24, 48, 72 and 168-hours lasting elicitation by two elicitors - substance A (N-(3-iodo-4-methylphenyl)pyrazine-2-carboxamide) and substance B (5-tert-butyl-6- chloro-N-(3-iodo-4-methylphenyl)pyrazine-2-carboxamide) on the isoflavonoids production in the callus cultures of Genista tinctoria. The cultures were cultivated on Schenk-Hildebrandt medium with addition of 0,5mg/l 2,4-dichlorophenoxyacetic acid a kinetine in concentration 0,1 mg/l. The isoflavonoids content was determined using HPLC. The maximal content of genistin was detected after 6-hours elicitation with substace A at concentration cA 2= 2,95 . 10-4 mol/l, the production was increased about 3200%. Within using substance B the maximal increase of genistin was detected after 12-hours lasting impact of concentration cB 1=2,33 . 10-3 mol/l (by 5640%). Generally higher production of isoflavonoids was proved when substance A was used.
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