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National Repository of Grey Literature

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NO Synthases, TGF-beta 1 and EGF Expression in Patients with Proven Oropharyngeal Helicobacter Pylori Infection and its Role in Oropharyngeal Pathogenesis Lukeš, Petr ; Astl, Jaromír (advisor) ; Šlapák, Ivo (referee) ; Fučíková, Terezie (referee) The thesis focuses on detection of Helicobacter pylori (HP) in the oropharyngeal lymphatic tissue and changes of selected cytokines expression according to the inflammatory response to the HP infection. HP is well known gastric pathogen, which leads to the development of chronic gastritis, gastric ulcers and malignancies such as gastric carcinoma or MALT lymphoma. According to the supposed way of transmission of this bacterium, oral cavity was investigated for HP presence recently. Data about possible colonisation of oropharyngeal tissue are insufficient. Several papers were published with inconsistent results. This inconsistency is due to different investigation methods used. The pathogenetic effect of HP in the oropharyngeal tissue was not investigated. It is supposed, that HP could lead to inflammation or carcinogenesis of oropharyngeal tissue in the same way as in gastric mucosa. The presence of HP in oropharyngeal tissue samples of patients suffering from tonsillar carcinoma, chronic tonsillitis or obstructive sleep apnea syndrome (OSAS) was investigated in this study. Real-time PCR was used. Part of the patients was investigated for gastric HP presence using UBT and PCR. Specific anti-HP antibodies and levels of EGF and TGF-beta 1 were detected in sera. Differences in iNOS, eNOS and caspase-3 were... Detailed record

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