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Enzyme hydrolysis of potato proteins and possibilities of fractionation of obtained peptide fragments
 MIKOVÁ, Klára
The diploma thesis is focused on enzyme hydrolysis of potato protein concentrates and fractionation of obtained peptide fragments. Were used protein concentrate from tubers variety Ornella and protein concentrate obtained by swedish company Lyckeby Starch AB. The enzyme hydrolysis lasted 24 hours and were used the proteolytic enzyme alkalasa and trypsin. In this work were prove possitive effect of enzyme hydrolysis on solubility and antioxidative properties of potato protein isolates. The fractionation of obtained peptide hydrolysated was based on systém FPLC (Fast protein liquid chromatography). The fractions contained of peptide fragments about 1, 350 kDa or fragments of smaller moleculary weight. The antixodative activity of subfractions were determIne by method called DPPH. The highest values (2,2 and 2,6 TEAC g/kg) were accured at the subfractions which were separations from Ornella hydrolyzates digeste by enzyme alkalasa.
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