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National Repository of Grey Literature

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Differences in fingerprints of biofilm-positive and biofilm-negative Candida strains exploitable for clinical practice Vykydalová, Marie ; Horká, Marie ; Šalplachta, Jiří ; Růžička, E. ; Kahle, Vladislav In this study, we have presented differences in fingerprints of Candida parapsilosis and Candida metapsilosis as well as their biofilm-positive and biofilm-negative forms. Fingerprints of yeasts were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis, capillary isoelectric focusing, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Samples for analyses were prepared by treatment of yeasts by boiling water or ethanol. Fingerprints of tested yeasts cells have differed in all cases. This knowledge can be used for obtaining rapid and simple method which distinguishes above mentioned yeasts in standard biochemical laboratories. Detailed record

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