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National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
Hydrolytic cleavage of coffee spent ground
 Skřivanová, Veronika ; Hlaváček, Viliam (referee) ; Obruča, Stanislav (advisor)
The bachelor thesis deals with various types of hydrolysis of spent coffee ground for the following biotechnological applications such as the culture medium. The work included mechanical, acid, alkaline and enzymatic hydrolysis. The resulting hydrolysates were analyzed concerning the content of reducing carbohydrates, and potentially inhibitory substances polyphenolic nature. The results show that the best conditions for culturing yeast samples were prepared with a concentration of coffee 150 g/l hydrolysed combination of 2,5% mineral acid by heating at 121 °C for 15 minutes. Using ultrasonic homogenizer significantly increases the effect of hydrolysis. The enzymatic hydrolysis is best placed hydrolyzate prepared cellulase. The hydrolysed spent coffee grounds were used as the production medium for the production of ethanol by Saccharomyces cerevisiae. For samples was determined concentration change of glucose and ethanol by HPLC/RID. The highest concentration of biomass sample contained hydrolyzed cellulose on the other side, the highest yields of ethanol (20,07 g/l) were achieve using hydrolysate prepared by 2,5% hydrochloric acid.
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