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Utilization of Light Scattering for the Study on the Structural and Conformation Changes during the Interaction of Biopolymers
Dobiášová, Andrea ; Hurčíková, Andrea (referee) ; Kalina, Michal (advisor)
The aim of this bachelor thesis was to study structural and conformation changes during the interaction of biopolymers by ligth scattering methods. This study was focused on hyaluronic acid with two molecular weights 250 – 450 kDa and 1500 – 1750 kDa, on sodium alginate and dextran hydrochloride (DEAE). NaCl, CaCl2 and BSA were used as interacting agents. Simple physical-chemical methods, like UV-VIS spectrometry, dynamic and electrophoretic light scattering, were used in experimental part. The main used method for these purposes was size exclusion chromatography coupled with light scattering and viscosimetry detectors. The studied characteristics of experiment were molecular weight, gyration and hydrodynamic radius, zeta potential and coefficients of conformation and Mark-Howinks diagrams. Interactions have been proved between negatively charged polyelectrolytes and additives. NaCl practically did not interact with any polysaccharide, only increasing ionic strength. Positively charged dextran interacted only with the BSA, despite having the same charge.
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Utilization of Light Scattering for the Study on the Structural and Conformation Changes during the Interaction of Biopolymers
Dobiášová, Andrea ; Hurčíková, Andrea (referee) ; Kalina, Michal (advisor)
The aim of this bachelor thesis was to study structural and conformation changes during the interaction of biopolymers by ligth scattering methods. This study was focused on hyaluronic acid with two molecular weights 250 – 450 kDa and 1500 – 1750 kDa, on sodium alginate and dextran hydrochloride (DEAE). NaCl, CaCl2 and BSA were used as interacting agents. Simple physical-chemical methods, like UV-VIS spectrometry, dynamic and electrophoretic light scattering, were used in experimental part. The main used method for these purposes was size exclusion chromatography coupled with light scattering and viscosimetry detectors. The studied characteristics of experiment were molecular weight, gyration and hydrodynamic radius, zeta potential and coefficients of conformation and Mark-Howinks diagrams. Interactions have been proved between negatively charged polyelectrolytes and additives. NaCl practically did not interact with any polysaccharide, only increasing ionic strength. Positively charged dextran interacted only with the BSA, despite having the same charge.
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