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National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
Microbial diversity of breast milk and its influence on the gut microbiome of infants
 Novotná, Jana ; Vítová, Eva (referee) ; Trachtová, Štěpánka (advisor)
Breast milk is considered the most important source of nutrition for infants and newborns due to its content of all the necessary nutrients and bioactive components that positively affect the child's immune system. The theoretical part of my work deals with the microbial composition of breast milk and its transformation depending on the stages of breastfeeding and other factors. The experimental part is focused on the identification of selected bacterial strains in breast milk and stool of a breastfed child and a child fed an artificial milk diet. Collection strains of bacteria belonging to the genera Bifidobacterium, Lactobacillus, and Candida were used for the analysis. DNA was isolated by phenol extraction, using magnetic carriers and a commercial kit. The presence of bacterial DNA was proved by q-PCR analysis using genus-specific primers for selected bacteria.
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