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The proteomic study of abiotic stress of plants.
Barabášová, Kamila ; Podlipná, Radka (advisor) ; Smrček, Stanislav (referee)
Keywords: Arabidopsis thaliana, phytoremediation, abiotic stress, ibuprofene, doxorubicin, two-dimensional electrophoresis Nowadays, develop of the pharmaceutical industry is very fast. Reason of this trend is ever-increasing number of diseases, lifestyle and still increasing demand for the drugs. With this trend growing interest in the analysis of the residues of pharmaceuticals in the environment which is result of incomplete wastewater treatment. This diploma thesis is studying effect of cytostatic drugs, specifically doxorubicin and one of the most widely used analgesics - ibuprofen, at the proteome level of the model plant Arabidopsis thaliana. Proteins isolated from plants exposed to the drugs were separated by two-dimensional electrophoresis. Comparing of protein maps by PDQest program (Bio-Rad, USA) was found several proteins whose expression was affected by the presence of drugs in the culture medium. Selected proteins were identified by LC - MS / MS.
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