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Optimization of calcium chloride concentration for removal of polysaccharide contamination during plant DNA isolation
Frnčová, Ekaterina ; Šlosárová, Katarína (referee) ; Fialová, Lenka (advisor)
The greatest difficulty in isolating DNA is the presence of contaminants that cause side effects. Polysaccharides are the most common contaminants in fruits. They can distort the results in spectrophotometric determination of purity or act as inhibitors in PCR analysis together with other substances (for example, proteins or phenolic substances). The aim of this work was to investigate the influence of different concentrations of calcium chloride on the process of DNA isolation. In the experimental part, DNA from the apple was isolated using different concentrations of calcium chloride. The isolation was carried out four times, and each time the sample was adjusted in different ways. It was found that the isolation method used works only with a sample that has been lyophilized. Isolation of DNA from fresh fruit provided very low yields. Probably, this was due to the large water content in the sample, and the proportion of the solid component was smaller. Subsequently, PCR analysis and electrophoresis were performed to determine the amplifiability of the isolated DNA. Two sets of primers with different specificity were used for this analysis. Amplifiability was confirmed only when using primers specific to apple DNA when using 100 mM solution of CaCl2. Other samples have been amplifiable using both types of primers. Probably, samples isolated using a 100 mM solution of CaCl2 had a larger amount of inhibitors that do not affect all PCR reactions equally, which may also indicate a small effectiveness of this amount of CaCl2.
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Optimization of the MRI chicken embryos measurements
Sedláčková, Gabriela ; Dražanová, Eva (referee) ; Krátká, Lucie (advisor)
This thesis deals with optimization of MRI measuring of phantoms containing the chicken embryo. The theoretical part of thesis is dedicated to the MRI fundamentals and description of pulse sequences. In the next part the options of keeping and monitoring the temperature are being discussed. The thesis also deals with design and realization of phantoms which are used for measuring. A browser of images captured by MRI was programmed in Matlab environment. The program allows users to look through the images with different settings of pulses sequences or kind of phantom. The final part of thesis describes the anatomical structures of chicken embryo and discussion of collected results.
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Use of energetic by-products and other secondary raw materials for the alpha gypsum production
Moldrzyk, Jan ; Jiroušková, Kateřina (referee) ; Dvořák, Karel (advisor)
Thesis focus on the longterm research in an area of alpha gypsum production by non pressure method in chloride salt solutions production, which is researched by Institute od technology of building materials and components, Faculty of civil engineering for many years. The result of given research is non pressure equipment for alpha gypsum production. The scope of this research was to make a production of alpha gypsum more efficient through modification of the facility itself and through an optimalization of dehydration process. The scientific aim of this process was to compare an influence of dehydratation solutions of CaCl2, NaCl and KCl on the final product provided that molarity of solutions was at the same level.
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Preparation of high performance sulphate binder by nonpressure method
Urban, Karel ; Novák, Jan (referee) ; Dvořák, Karel (advisor)
Institut of Technology building materials and components in FAST VUT Brno is for several years engageding by research of unpressurized production of high sulphate binders and also by developing a dehydrating apparatus. This work follows on this research. The main aim of this work is to prepare the alpha calcium sulfate hemihydrate by method of dehydration in the various solutions the chloride salts of the same molal concentration in an innovative dehydration apparatus. Another aim is monitoring the influence dehydrating solutions on the basis of KCl, NaCl and CaCl2 on the properities of made gypsum.
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Use of energetic by-products and other secondary raw materials for the alpha gypsum production
Moldrzyk, Jan ; Jiroušková, Kateřina (referee) ; Dvořák, Karel (advisor)
Thesis focus on the longterm research in an area of alpha gypsum production by non pressure method in chloride salt solutions production, which is researched by Institute od technology of building materials and components, Faculty of civil engineering for many years. The result of given research is non pressure equipment for alpha gypsum production. The scope of this research was to make a production of alpha gypsum more efficient through modification of the facility itself and through an optimalization of dehydration process. The scientific aim of this process was to compare an influence of dehydratation solutions of CaCl2, NaCl and KCl on the final product provided that molarity of solutions was at the same level.
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Optimization of the MRI chicken embryos measurements
Sedláčková, Gabriela ; Dražanová, Eva (referee) ; Krátká, Lucie (advisor)
This thesis deals with optimization of MRI measuring of phantoms containing the chicken embryo. The theoretical part of thesis is dedicated to the MRI fundamentals and description of pulse sequences. In the next part the options of keeping and monitoring the temperature are being discussed. The thesis also deals with design and realization of phantoms which are used for measuring. A browser of images captured by MRI was programmed in Matlab environment. The program allows users to look through the images with different settings of pulses sequences or kind of phantom. The final part of thesis describes the anatomical structures of chicken embryo and discussion of collected results.
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Preparation of high performance sulphate binder by nonpressure method
Urban, Karel ; Novák, Jan (referee) ; Dvořák, Karel (advisor)
Institut of Technology building materials and components in FAST VUT Brno is for several years engageding by research of unpressurized production of high sulphate binders and also by developing a dehydrating apparatus. This work follows on this research. The main aim of this work is to prepare the alpha calcium sulfate hemihydrate by method of dehydration in the various solutions the chloride salts of the same molal concentration in an innovative dehydration apparatus. Another aim is monitoring the influence dehydrating solutions on the basis of KCl, NaCl and CaCl2 on the properities of made gypsum.
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