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National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
Utilization of polymerase chain reaction, PCR-RFLP technique and sequence analysis for determination of homozygotes, intraspecific heterozygotes and interspecific hybrids of the genus Salvelinus in breeding facilities
 Mendel, Jan ; Halačka, Karel ; Mareš, J.
The methodology provides relevant information and reliable instruments for determination of the most frequently bred fish of the genus Salvelinus in the Czech Republic and in European countries. It includes the optimized process of identification of four species (Salvelinus fontinalis, Salvelinus alpinus, Salvelinus umbla, Salvelinus namaycush) in the homozygous and heterozygous state and in their hybrid combinations. The methodology describes the present situation in identification of the above mentioned fish species in the Czech Republic including its drawbacks and at the same time proposes a new and reliable solution utilizing polymerase chain reaction, PCR-RFLP restriction technique and sequencing. Visualization and separation of diagnostic fragments is performed either on agarose gel or on the Fragment AnalyzerTM.
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