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Analysis of main wine saccharides
Horáková, Hana ; Šabata, Pavel (referee) ; Vespalcová, Milena (advisor)
This diploma thesis deals with determination of carbohydrates in wine. Theoretical part attends to production of wine from grape to treatment and training of wine. It refers saccharides in wine, especially glucose and fructose. The study provides an overview of the available sources concerning the possibilities of determination of carbohydrates in wine by chromatographic methods and Skalar automated chemistry analyser. The study shortly refers simple analytical methods for determination of wine saccharides. The experimental part based on this search deals with analysis of saccharides by high performance liquid chromatography with refractive index detector and UV detector on aminoalkyl column and determination of reducting sugars by Skalar automated chemistry analyser. Finally, the results of these methods were compared.
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Stanovení vybraných obsahových látek ve vínech metodou kapalinové chromatografie
Hermanová, Veronika
This thesis deals with the analysis of wines using high-pressure liquid chromatography in order to analyze the content of alcohols, organic acids, and carbohydrates in wine. The most important groups and their representatives are presented in the literary review, as well as the theoretical basis of chromatographic methods, selected types of detectors, and used stationary and mobile phases. In the experimental part, analytical parameters of wines from the retail network, laboratory-prepared must and wines prepared by fermentation were measured using liquid chromatography methods with refractometric and UV detection. The results indicate the suitability of this combination of detectors for the selected wine analyses.
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Capillary electrophoresis with dual optical and contactless conductometric detection.
Kadlecová, Tereza ; Opekar, František (advisor) ; Jelínek, Ivan (referee)
This work deals with dual detection of organic and inorganic analytes after separation by capillary zone electrophoresis. In the first part, two types of hydrodynamic sampling are tested. Standard hydrodynamic sampling most often used in laboratory-made electrophoretic apparatus, based on lifting the vessel with the sample, in which the sampling end of the capillary is immersed, and a new method based on increasing the pressure in the sampling vessel without moving the capillary. This sampling procedure minimizes experimenter activity because it is controlled by software. Experimenter only changes vessel containing the sample solution for one with separation electrolyte. The experimental parameters, the sampling time and pressure, are optimized to achieve maximum separation efficiency and adequate detection sensitivity. In the second part of the work, the developed method is tested for the separation of amino-acids in a biological sample (urine).
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Development of UV detector for space applications
Kočiščák, Samuel ; Pavlů, Jiří (advisor) ; Jeřáb, Martin (referee)
Title: Development of UV Detector for Space Applications Author: Samuel Kočiščák Department: Department of Surface and Plasma Science Supervisor: doc. RNDr. Jiří Pavlů, Ph.D., Department of Surface and Plasma Science Abstract: It is not unusual that space measurement is influenced by UV irradia- tion causing photoemission on delicate electronic components. Therefore informa- tion on UV irradiance is very useful. Particularly useful is non-delayed, immediate quantitative information (on a time scales of seconds) identifying unexpected va- riation in the irradiance of such electronic parts. The goal was to construct a prototype, measure basic properties and discuss ca- pabilities of a detector capable of continuous measurement of UV irradiance of a spacecraft, working on the principle of photoemission alone. We have success- fully shown the capabilities of a prototype and established some of its key basic properties, e.g. their stability in cosmic environment. Keywords: VUV, Faraday cup, UV detector, UV transmittance, solar wind
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Development of UV detector for space applications
Kočiščák, Samuel ; Pavlů, Jiří (advisor) ; Jeřáb, Martin (referee)
Title: Development of UV Detector for Space Applications Author: Samuel Kočiščák Department: Department of Surface and Plasma Science Supervisor: doc. RNDr. Jiří Pavlů, Ph.D., Department of Surface and Plasma Science Abstract: It is not unusual that space measurement is influenced by UV irradia- tion causing photoemission on delicate electronic components. Therefore informa- tion on UV irradiance is very useful. Particularly useful is non-delayed, immediate quantitative information (on a time scales of seconds) identifying unexpected va- riation in the irradiance of such electronic parts. The goal was to construct a prototype, measure basic properties and discuss ca- pabilities of a detector capable of continuous measurement of UV irradiance of a spacecraft, working on the principle of photoemission alone. We have success- fully shown the capabilities of a prototype and established some of its key basic properties, e.g. their stability in cosmic environment. Keywords: VUV, Faraday cup, UV detector, UV transmittance, solar wind
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Capillary electrophoresis with dual optical and contactless conductometric detection.
Kadlecová, Tereza ; Opekar, František (advisor) ; Jelínek, Ivan (referee)
This work deals with dual detection of organic and inorganic analytes after separation by capillary zone electrophoresis. In the first part, two types of hydrodynamic sampling are tested. Standard hydrodynamic sampling most often used in laboratory-made electrophoretic apparatus, based on lifting the vessel with the sample, in which the sampling end of the capillary is immersed, and a new method based on increasing the pressure in the sampling vessel without moving the capillary. This sampling procedure minimizes experimenter activity because it is controlled by software. Experimenter only changes vessel containing the sample solution for one with separation electrolyte. The experimental parameters, the sampling time and pressure, are optimized to achieve maximum separation efficiency and adequate detection sensitivity. In the second part of the work, the developed method is tested for the separation of amino-acids in a biological sample (urine).
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Analysis of main wine saccharides
Horáková, Hana ; Šabata, Pavel (referee) ; Vespalcová, Milena (advisor)
This diploma thesis deals with determination of carbohydrates in wine. Theoretical part attends to production of wine from grape to treatment and training of wine. It refers saccharides in wine, especially glucose and fructose. The study provides an overview of the available sources concerning the possibilities of determination of carbohydrates in wine by chromatographic methods and Skalar automated chemistry analyser. The study shortly refers simple analytical methods for determination of wine saccharides. The experimental part based on this search deals with analysis of saccharides by high performance liquid chromatography with refractive index detector and UV detector on aminoalkyl column and determination of reducting sugars by Skalar automated chemistry analyser. Finally, the results of these methods were compared.
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