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Production, characterization and application of polymers of malic and glutamic acids
Čangelová, Katarína ; Kučera, Dan (referee) ; Obruča, Stanislav (advisor)
Subject of this bachelor thesis was study of biopolymers production using microorganisms. Polymer of malic acid was produced by Aureobasidium pullulans, a yeast-like fungus. Biosynthesis of polymer of glutamic acid was carried out by Bacillus licheniformis bacterium. Particular polymers, their microbial producers and possible application in different areas are characterised in the theoretical part of the thesis. Through experiments, the effect of various conditions on cell growth and production of these polymers was studied. Cultivation conditions were optimised in terms of polymers production. Subsequently, waste substrates - whey and poultry feathers were used. Microbially-produced polymer of malic acid was hydrolysed with 2 M sulfuric acid. Concentration of malic acid was analysed by IEC with a conductivity detector. Concentration of polymer of glutamic acid was determined by a spectrophotometric method using CTAB.
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Production, characterization and application of polymers of malic and glutamic acids
Čangelová, Katarína ; Kučera, Dan (referee) ; Obruča, Stanislav (advisor)
Subject of this bachelor thesis was study of biopolymers production using microorganisms. Polymer of malic acid was produced by Aureobasidium pullulans, a yeast-like fungus. Biosynthesis of polymer of glutamic acid was carried out by Bacillus licheniformis bacterium. Particular polymers, their microbial producers and possible application in different areas are characterised in the theoretical part of the thesis. Through experiments, the effect of various conditions on cell growth and production of these polymers was studied. Cultivation conditions were optimised in terms of polymers production. Subsequently, waste substrates - whey and poultry feathers were used. Microbially-produced polymer of malic acid was hydrolysed with 2 M sulfuric acid. Concentration of malic acid was analysed by IEC with a conductivity detector. Concentration of polymer of glutamic acid was determined by a spectrophotometric method using CTAB.
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Data Source Analysis in State Administration
Rezek, Martin ; Ulman, Miloš (advisor) ; Martin, Martin (referee)
This diploma thesis tackles the issue of data sources used in state administration. Its
aim is to carry out a process analysis of data sources used in internal processes within the
Czech Social Security Administration authority (Česká správa sociálního zabezpečení) and
propose changes to optimise processes within the institution. The theoretical part of the
thesis defines communication between a citizen and public administration and describes its
basic elements. It also looks at the Process Management In Public Administration project
and the evaluation of applications for new ICT projects.
The practical part deals with the application of the theoretical findings to processes
within a particular body of the state administration, the CSSA. The resulting proposal to
optimise selected processes within the entire CSSA institution is based on a process analysis
of data sources from a branch of the District Social Security Administration office (Okresní
správa sociálního zabezpečení) as well as two case studies.
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