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Molecular genetic characterization of the rare tumours of the urogenital tract.
Šteiner, Petr ; Vaněček, Tomáš (advisor) ; Španielová, Hana (referee)
The aim of this study was molecular characterization of four types of renal tumours (papillary renal cell carcinoma [PRCC], tubulocystic renal carcinoma [TCRC], pseudorossette forming renal carcinoma [PRRC] and unclassified renal carcinomas [URC]) and two types of rare tumours of the testes (Adult type of granulosa cell tumours [ATGCTs] and Incompletely differentiated sex cord stromal tumours [ISCSTs]). In case of TCRC the activity of signalling pathways involved in angiogenesis was studied. The aim was to determine the suitability of antiangiogenic agents for treatment of TCRC. Next, the methylation profile of 24 tumor suppressor genes was studied in TCRC and PRCC in order to analyze their similarity. Eventual differences could be helpful tool in differential diagnostics. Also, spectrum of chromosomal aberrations was analyzed by array-CGH in one case of PRRC and two cases of URC. Any unique aberration found would be useful in differential diagnostics of these tumors. Last, but not least, the specificity of mutation c.402C>G of FOXL2 gene for ovarian ATGCTs was verified by studying its occurrence in testicular ATGCTs and ISCSTs. Analysis of mRNA levels did not reveal any enhanced activity of the studied signalling pathways. Cluster analysis of methylation profiles showed close relationship between PRCC a...
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Mechanisms of gonad determination and differentiation in reptiles
Ehl, Jan ; Kratochvíl, Lukáš (advisor) ; Krylov, Vladimír (referee)
Reptiles, as a group, have a~lot of various types of sex-determining mechanisms. Evolution of sex- deter- mining mechanisms took place in different groups of reptiles by different way and got to different types of sex determination. Gene network underlying gonadal diferenciation has been investigated in many studies, for revealing of principles causing this diversity. Thanks to that, it is very difficult to determine ultimate trigger of switching between male of female fate.Interesting fenomenon is sexreversal, which is enabled by mechanism activated only by unusual circumstances. I have summerized basic facts about sex determination in reptiles in this work. Also, expresion patterns of some genes important for gonadal diferenciation in vertebrates, are recapitulated there. The last things, what this work posses, is summary of knowledge about sex-reversal in reptiles and short treatise about its evotution.
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Molecular genetic characterization of the rare tumours of the urogenital tract.
Šteiner, Petr ; Vaněček, Tomáš (advisor) ; Španielová, Hana (referee)
The aim of this study was molecular characterization of four types of renal tumours (papillary renal cell carcinoma [PRCC], tubulocystic renal carcinoma [TCRC], pseudorossette forming renal carcinoma [PRRC] and unclassified renal carcinomas [URC]) and two types of rare tumours of the testes (Adult type of granulosa cell tumours [ATGCTs] and Incompletely differentiated sex cord stromal tumours [ISCSTs]). In case of TCRC the activity of signalling pathways involved in angiogenesis was studied. The aim was to determine the suitability of antiangiogenic agents for treatment of TCRC. Next, the methylation profile of 24 tumor suppressor genes was studied in TCRC and PRCC in order to analyze their similarity. Eventual differences could be helpful tool in differential diagnostics. Also, spectrum of chromosomal aberrations was analyzed by array-CGH in one case of PRRC and two cases of URC. Any unique aberration found would be useful in differential diagnostics of these tumors. Last, but not least, the specificity of mutation c.402C>G of FOXL2 gene for ovarian ATGCTs was verified by studying its occurrence in testicular ATGCTs and ISCSTs. Analysis of mRNA levels did not reveal any enhanced activity of the studied signalling pathways. Cluster analysis of methylation profiles showed close relationship between PRCC a...
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