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Přenos významných patogenů aktivovaný klíštěcími slinami
HUSPEKOVÁ, Helena
Ticks are one of the most common ectoparasites in the world and they are vectors of many tick-borne diseases. These diseases are caused by pathogens, including bacteria and viruses. The main point of the thesis is focused on enhancement of pathogens transmission, (especially Borrelia burgdorferi sensu lato, the causative agent of Lyme disease) by tick saliva or salivary glands extracts, and I tried to refer the main molecules, which have immunomodulatory or inhibition effect on the immunity of host. This effect can lead to the enhancement of pathogens transmission and contributes to their extension in host. The second part of the thesis is based on molecules, which can lead to the creation of vaccines against tick-borne diseases.
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Detekce spirochét lymské boreliózy v klinických vzorcích metodami PCR a optimalizace podmínek kultivace borelií ze vzorků pacientů s příznaky lymské boreliózy
HAVRAN, Jiří
The samples under investigation were collected in Department of Pediatric Infectious Diseases of the University Hospital (Brno). Group of patients (100) was heterogeneous in terms of symptoms, age and sex. The samples were taken from patients with an LB diagnosis and from those with nonspecific symptoms. Molecular typing of LB spirochetes in clinical samples (104 blood/serum, 89 cerebro-spinal fluid and 1 synovial fluid) became necessary when the general immunological tests gave unclear results. The samples were analyzed using PCR-based and molecular biology techniques that include: nested- and spacer-PCR, specie-specific PCR, sequence, virtual hybridization, in silico RFLP analysis, similarity search. Results of conducted analysis confirmed that 51% of samples (98) were positive on B. bugrdorferi sensu lato. Using above mentioned techniques 6 spirochete species from B. burgdorferi sensu lato complex were identified; two of them weren?t detected in samples of human origin in Europe yet. Comparative analysis of two media for Borrelia cultivation from samples of human origin definitely proved the adventage of using MKP instead of traditionally used BSK-H Complete.
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Vliv vakcinace slinami klíštěte \kur{Ixodes ricinus} na přenos \kur{Borrelia burgdorferi} a cytokinový profil hostitele
GREGOROVÁ, Eva
Klíště Ixodes ricinus je jedním z nejvýznamnějších přenašečů patogenů napadajících člověka jako jsou spirochety Borrelia burgdorferi a virus TBE. V této práci byl studován vliv opakovaného sání neinfikovaných nymf I. ricinus a vliv vakcinace slinami klíštěte I. ricinus na přenos Borrelia burgdorferi a produkci cytokinů. Regionální lymfatické uzliny myší C3H/HeN byly vyšetřovány na produkci IL-2, IFN-? a IL-10 imunoenzymatickou metodou ELISA. Množství spirochet v kůži, srdci a močovém měchýři bylo stanovováno kvantitativní PCR v reálném čase.
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