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Comparison of mitochondrial DNA for species identification
Labounek, René ; Provazník, Ivo (referee) ; Maděránková, Denisa (advisor)
The work deals with the method of recognizing species on the analysis of mitochondrial DNA segment. This analysis and classification using segment gene called CO1 in literatures such as barcode of life. In the beginning of work is analyzed the mitochondrial theory of heredity and conditions of formation of barcode. Practical use is based on this theory in creating database of barcodes generated to different animal species. Data used for creating the library are drawn from public databases NCBI and BOLD Systems. The next part of this work concerns about methods of comparison of the individual barcodes to the others and especially to the barcode of human. Three main computing methods were used tore these analyses: Needleman-Wunsch algorithm, Smith- Waterman algorithm and comparison of similarities using distance matrix. This work also concerns about transformation of DNA molecule sequences from symbols to numeric formats, which is required for the distance matrix comparison method. Algorithms for searching for a barcode of a species and vice versa were created to ease the work with data.
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Genetic diversity of chosen horse breeds
JANKOVSKÁ, Tereza
The objective of the thesis is to clarify the findings about genetical biodiversity of horses, evaluation of the diversity and description of current state of genetic resources preservation. Firstly, the history is described, domestication of horses, breeds and biodiversity. From the genetic point of view, the thesis contains description of the genetical diversity, microsatellites, markers and mitochondria. There are described genetic sources and their preservation. Current quantities and thein progress are also mentioned.
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Assembly and annotation of a mitochondrial genome of kinetoplastid protist \kur{Perkinsela}.
DAVID, Vojtěch
This thesis is based on a collaborative project between Laboratory of Molecular Biology of Protists led by Prof. Julius Lukeš and the laboratory of John Archibald (Department of Biochemistry & Molecular Biology, Dalhousie University, Canada). My contribution to the project, as the name of the thesis suggests is to process DNA and RNA sequencing data from Neoparamoeba pemaquidensis isolate that correspond to the mitochondrion of Perkinsela, also called Ichthyobodo-related organism (IRO). The main aim is to characterize mitochondrial gene content and to attempt to understand the mechanism of U-insertion/deletion RNA editing in Perkinsela and its evolution.
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