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Comparison of level total cholesterol in serum for residents from Pilsen and Czech Budweis provided similar eating habits
FRAITOVÁ, Lucie
Measuring cholesterol is considered as a screening test used primarily to determine cardiovascular risk and monitoring lipid-lowering therapy. Since this is a screening examination, there is a need for further laboratory tests - the lipid profile (HDL cholesterol, LDL cholesterol, triglycerides). Patients who get examined as a part of lipid-lowering therapy should follow a diet. These patiens need to be examined more frequently. Population of Czech Republic is considered at risk of early occurrence of atherosclerosis and its complications. Every year in the Czech Republic nearly 60 000 people die from cardiac and vascular disease. Cardiovascular disease increasingly affects younger age group. They are also a cause of disability of people of working age. One of the main indicators is high level of cholesterol. Accurate and precise measurement of blood cholesterol plays a role in the reduction of morbidity and mortality of people with cardio vascular disease. The aim of this thesis is to: 1) Measure the total cholesterol sample population of Plzeň and České Budějovice 2) Compare and find differences in serum total cholesterol in both locations In the theoretical part of the thesis I was interested in what foods increase cholesterol and what foods can reduce high cholesterol. Furthermore, the theoretical part describes diseases that may occur during hypercholesterolemia. The basic ones include atherosclerosis and its complications, which include heart disease or stroke. Preanalytical factor that may significantly affect laboratory tests are described in this part too. The practical part describes everything from an intake of biological material in the laboratory through the preparation (centrifugation, creating aliquots) to insertion of a secondary sample into the analytical device. Research for the thesis was conducted in the biochemical laboratory of synlab czech s.r.o. in České Budějovice. In this part I investigate ? under supervision ? a total of 100 samples of venous blood from people of Plzeň and České Budějovice. I determined the total cholesterol in serum. Cobas Integra 800 automatic biochemical analyzer was used for the examination of the biological material. In routine practice enzymatic methods are used to determine levels of cholesterol. Cholesterol esters are converted by cholesterol esterase into free cholesterol and fatty acids. Free cholesterol is oxidized by cholesterol oxidase to form cholestenone and hydrogen peroxide. The resultant hydrogen peroxide reacts with 4-aminoantipyrine and phenol to form a red color and water. The color intensity is directly proportional to the concentration of cholesterol and is measured by absorption spectrophotometry at 512 nm.Measured laboratory results were statistically processed and neatly sorted into tables and graphs using the computer program. Data from Plzeň and České Budějovice were sorted according to gender and evaluated using graphs. The data sets were then compared to each other and the difference in cholesterol levels was registered. All the results are in the table and graphs. Hypercholesterolemia was detected in 29 samples from Plzeň and 27 from České Budějovice.
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The role of nurses in the primary prevention of cardiovascular diseases
CICHROVÁ, Lenka
The thesis seeks to examine the role of nurses in primary prevention of cardiovascular diseases (CVD). Today, CVD are one of the most frequent causes of death in adults worldwide. In Europe they are involved in up to 40% of deaths annually. These diseases affect the heart and blood vessels and can occur in several forms, such as ischemic heart disease, stroke or high blood pressure. Therefore, primary prevention is a vital part of nursing interventions. Prevention of CVD is closely linked to the awareness of risk factors. Hence, it should be aimed at proper handling of such factors or possible treatment. The work is composed of two parts, the first part being theoretical and the second practical. Two objectives were set for this thesis. Firstly, to map out the role of nurses in primary prevention of cardiovascular diseases; secondly, to determine whether nurses in GP surgeries are involved in primary prevention of cardiovascular diseases. The following research questions were defined: ?What is the main role of nurses in primary prevention of cardiovascular diseases???How do nurses in GP surgeries engage in primary prevention of cardiovascular diseases???Do nurses in GP surgeries use the SCORE risk charts??The qualitative research revealed that nurses are most often involved in the prevention of CVD as proper lifestyle educators and monitors of blood pressure, cholesterol and blood glucose. It was also found that nurses in GP surgeries engage in primary prevention of CVD. The thesis records interviews carried out with nurses, describing the interviews in the results. The most common and most interesting responses are given in tables. The results of the thesis can be used as a source of information on proper primary prevention of CVD in GP surgeries. This information was used to create guidelines for nurses on how to proceed in the primary prevention of CVD.
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Creation and verification of the tutorial: Health education and prevention of the non-infectious disorders (ischemic heart disease)
HABARTOVÁ, Silvie
This thesis introducestheimportanceofpreventionofcardiovasculardisease. Itprovides a brieflookatthe anatomy and physiologyoftheheart and bloodvessels. Gives basic informationaboutcoronaryheartdisease, causes, symptoms, diagnosis, treatment and rehabilitationofpatientswiththisdisease. Itrepresents a multifactorialdiseaseatherosclerosis. Itrecallsthebasicsoffirstaid. Thegoalis to create a training program for full-timestudents and therebyincreasetheawarenessofstudents on theimportanceofpreventingcardiovasculardiseases. Thepractical part ofthe thesis, provides evidence ofknowledgeofstudentscontinuingstudiesofthe major risk factorsofcardiovasculardiseases and theirprevention.
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Influence of increased aplication of the file polyunsaturated fatty acids on alteration of level cholesterol of individuum.
FLANDERKOVÁ, Michaela
I have engaged in the influence of increased aplication of the file polyunsaturated fatty acids on alteration of level cholesterol of individuum in my bachelory work. At first I had studied theoretical knowledge focused the theme of polyunsatureted fatty acid and cholesterol. I had devoted to this knowledge in theoretical part of my bachelory work. After I had contacted some people. I had known that they have increased cholesterol level. This people I had acquainted with themes of my bachelory work and development of research. The acquainted people have accepted part of research. Initial measuring taked place after. The measuring determined the level of total cholesterol, LDL cholesterol, HDL cholesterol, triacylglycerol. The three month interventionist program taked place after, from march 2011 to december 2012. The Participant of research had consumed of food supplement, which contented fish oil. Fish oil is rich in content of polyunsaturated fatty acid omega 3 (EPA and DHA). Output measuring taked place after three month. On the basis of measuring got results. I worked with results in the practical part of my bachelory work. I have compared results of initial and output measuring.
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