National Repository of Grey Literature 12 records found  previous11 - 12  jump to record: Search took 0.01 seconds. 
Utilisation of phase contrast in X-ray computed tomography
Kalasová, Dominika ; Švéda,, Libor (referee) ; Zikmund, Tomáš (advisor)
X-ray computed tomography is a nondestructive method for 3D imaging of inner structure of objects; it is, however, restricted by absorption properties of materials. With phase contrast imaging, observation of samples with low absorption or with parts with similar absorption becomes possible. For a long time, phase contrast imaging has been restricted to synchrotron radiation sources or special techniques due to requirement of spatial coherence of radiation. Along with recent development of X-ray micro and nanofocus tubes and X-ray detectors a phase contrast imaging becomes available also with laboratory sources. In this work an overview of phase contrast imaging methods is given, with an emphassis on propagation based method. Examples of this method and application of phase retrieval algorithm on samples from RIGAKU Nano3DX and GE phoenix v|tome|x L240 stations within Laboratory of X-ray micro and nanotomography CEITEC BUT are shown.
Methodology of Dictyostelium discoideum imaging with the transmission digital holographic microscope
Křížová, Aneta ; Ježek, Jan (referee) ; Uhlířová, Hana (advisor)
The task of this bachelor’s thesis was to image Dictyostelium discoideum with a transmission digital holographic microscope and compare this imaging with a commonly used microscopy technique. This bachelor's thesis is structured into a theoretical part and an experimental part. The theoretical part describes the setting of the transmission digital holographic microscope, basic operation and physical principles of imaging with this microscope. Further the process of image reconstruction and numerical process of phase reconstruction is illustrated. At the end of this part, basic biological characteristics of observed cells of Dictyostelium discoideum are introduced. The experimental part describes the cultivation and preparation of Dictyostelium discoideum for the observations, and further the process of performed experiments. The cells of Dictyostelium discoideum have been imaged in couple experiments by a method of the classical phase contrast. By the transmission digital holographic microscope the cells of Dictyostelium discoideum have been imaged in two mediums. Images captured by the transmission digital holographic microscope were processed by a method of Dynamic phase differences and analysed.

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